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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [RNA-Seq]

ABSTRACT: Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [RNA-Seq]

PROVIDER: PRJNA535506 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8956730.fastq.gz	Fastqsanger.gz
	SRR8956740.fastq.gz	Fastqsanger.gz
	SRR8956750.fastq.gz	Fastqsanger.gz
	SRR8956731.fastq.gz	Fastqsanger.gz
	SRR8956741.fastq.gz	Fastqsanger.gz

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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression

Project description:Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression

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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [ATAC-Seq]

Project description:Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [ATAC-Seq]

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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [ATAC-Seq]

Project description:Pygopus2 (Pygo2), a co-activator of Wnt/ β-catenin signaling can also bind H3K4me2/3 and participate in chromatin reading and writing. To investigate the functional relevance of histone binding activity of Pygo2 in malignant progression of breast cancer, we generated a knockin mouse model wherein binding of Pygo2 to H3K4me2/3 was rendered ineffective. Loss of Pygo2-histone binding resulted in smaller, differentiated and less metastatic tumors, at least in part due to a decrease in Wnt/ b-catenin signaling. RNA and ATAC-Seq analyses of tumor-derived cell lines revealed a downregulation of TGFb signaling and an upregulation of differentiation pathways like PDGFR. This also correlated with an increase in luminal cell fate which could be rescued by inhibition of the PDGFR pathway. Mechanistically, we find that Pygo2-histone interaction is important for expression of mir-29 family members to potentiate Wnt/ b-catenin signaling by suppressing Wnt signaling antagonists expression in addition to suppression of PDGFR expression. Collectively, our results demonstrate that histone binding function of Pygo2 is important for driving de-differentiation and malignancy of breast tumors and loss of this binding activates differentiation pathways that lead to decreased tumor growth and metastases. Ablating the Pygo2-H3K4me3 interaction hence might serve as an attractive therapeutic target for breast cancer.

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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression [RNA-Seq]

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Genome wide analysis of important transcription factors and histone modifications for prostate cancer progression

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Pygopus2-Histone interaction is important for de-differentiation and breast cancer progression

Project description:This SuperSeries is composed of the SubSeries listed below.

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Genome wide analysis of important transcription factors and histone modifications for prostate cancer progression

Project description:Prostate cancer is the most common cancer in men and androgen receptor (AR) downstream signalings promote prostate cancer progression. Although androgen deprivation therapy is effective for treating prostate cancer, most tumors relapsed as castration-resistant prostate cancer (CRPC). We performed ChIP-seq analysis to investigate the role of important transcription factors and histone modifications using AR positive prostate cancer cells, LNCaP, CRPC model cells, 22Rv1 and DU145 cells.

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A Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT

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