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Methylomonas methanica

ABSTRACT: Methylotrophic bacterium

PROVIDER: PRJNA53955 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR071390_1.fastq.gz	Fastqsanger.gz
	SRR071390_2.fastq.gz	Fastqsanger.gz
	SRR3926901_1.fastq.gz	Fastqsanger.gz
	SRR3926901_2.fastq.gz	Fastqsanger.gz

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Complete genome sequence of the aerobic marine methanotroph Methylomonas methanica MC09.

Project description:Methylomonas methanica MC09 is a mesophilic, halotolerant, aerobic, methanotrophic member of the Gammaproteobacteria, isolated from coastal seawater. Here we present the complete genome sequence of this strain, the first available from an aerobic marine methanotroph.

| S-EPMC3232845 | biostudies-literature

Methylomonas methanica MC09

Project description:: Complete genome sequence of the aerobic marine methanotroph Methylomonas methanica MC09

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Catalytic and Spectroscopic Properties of the Halotolerant Soluble Methane Monooxygenase Reductase from Methylomonas methanica MC09.

Project description:The soluble methane monooxygenase receives electrons from NADH via its reductase MmoC for oxidation of methane, which is itself an attractive C1 building block for a future bioeconomy. Herein, we present biochemical and spectroscopic insights into the reductase from the marine methanotroph Methylomonas methanica MC09. The presence of a flavin adenine dinucleotide (FAD) and [2Fe2S] cluster as its prosthetic group were revealed by reconstitution experiments, iron determination and electron paramagnetic resonance spectroscopy. As a true halotolerant enzyme, MmoC still showed 50 % of its specific activity at 2 M NaCl. We show that MmoC produces only trace amounts of superoxide, but mainly hydrogen peroxide during uncoupled turnover reactions. The characterization of a highly active reductase is an important step for future biotechnological applications of a halotolerant sMMO.

| S-EPMC9305295 | biostudies-literature

Crucial Role of the Chaperonin GroES/EL for Heterologous Production of the Soluble Methane Monooxygenase from Methylomonas methanica MC09.

Project description:Methane is a widespread energy source and can serve as an attractive C1 building block for a future bioeconomy. The soluble methane monooxygenase (sMMO) is able to break the strong C-H bond of methane and convert it to methanol. The high structural complexity, multiplex cofactors, and unfamiliar folding or maturation procedures of sMMO have hampered the heterologous production and thus biotechnological applications. Here, we demonstrate the heterologous production of active sMMO from the marine Methylomonas methanica MC09 in Escherichia coli by co-synthesizing the GroES/EL chaperonin. Iron determination, electron paramagnetic resonance spectroscopy, and native gel immunoblots revealed the incorporation of the non-heme diiron centre and homodimer formation of active sMMO. The production of recombinant sMMO will enable the expansion of the possibilities of detailed studies, allowing for a variety of novel biotechnological applications.

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Methylomonas methanica strain:S-1

Project description:Methylomonas methanica S-1 genome sequencing

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Methylomonas methanica strain:NCIMB 11130

Project description:Methylomonas methanica strain:NCIMB 11130 Genome sequencing and assembly

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Methylomonas methanica strain:R-45363

Project description:Methylomonas methanica strain:R-45363 Genome sequencing and assembly

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Methylomonas methanica strain:R-45371

Project description:Methylomonas methanica strain:R-45371 Genome sequencing and assembly