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Gene expression in human adipose tissue derived stromal cells (ADSC) after exposure to hypoxia

ABSTRACT: Gene expression in human adipose tissue derived stromal cells (ADSC) after exposure to hypoxia

PROVIDER: PRJNA539977 | ENA |

REPOSITORIES: ENA

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Gene expression in human adipose tissue derived stromal cells (ADSC) after exposure to hypoxia

Project description:Analysis of mRNA expression in ADSCs exposed to severe hypoxia. Hypothesis tested that hypoxia stimulates the expression of angiogenesis and neurogenesis-related genes in cultured ADSC. These data provide important information allowing to explain the role of ADSC in the stimulation of tissue regeneration

2019-04-27 | GSE130393 | GEO

Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells

Project description:Analysis of 20S proteasome in various tissue, adipose derived stem cells(ADSC) or HeLa cells treated with interferon-gamma. This results are compared with LC-SRM results obtained on the same samples. Global proteomics on ADSC amplified in 5% or 20% O2.

2019-02-14 | PXD011894 | Pride

RNAseq for subpopulations of adipose tissue stromal cells from mouse inguinal adipose tissue

Project description:We performed bulk RNA sequencing of several subpopulations of adipose tissue stromal cells to better understand the function of preadipocyte subpopulation (P1 and P2) in mouse inguinal adipose tissue. P1 and P2 cells isolated from inguinal adipose tissue from male and female C57/B6 mice, collected by FACS. P1 cells are CD31-CD45-TER119-SCA1+CD55+VAP1-CD142- cell population, and P2 cells are CD31-CD45-TER119-SCA1+CD55-VAP1+CD142- cell population. 50,000 cells were collected for each populations.

2021-12-07 | E-MTAB-9827 | biostudies-arrayexpress

Hypoxia exposure and methylation

Project description:Hypoxia exposure and methylation

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Comparison between stromal vascular fraction of adipose tissue, testis and epididymis in mouse

Project description:An experiment was performed in order to compare directly profiles of stromal vascular fraction of adipose tissue, testis and epidydimis, in order to rule out a possible contamination of the stromal vascular fraction of adipose tissue (SVF) by adjacent tissues (i.e. epididymis and testis). This led to the identification of a list of 2358 SVF-specific probes, which was subsequently used for further investigations. 3 dye-swap pairs, comparing 3 distinct samples of total RNA prepared from: (1) stromal vascular fraction of adipose tissue, (2) testis and (3) epidydimis.

2010-05-31 | E-GEOD-16613 | biostudies-arrayexpress

The effects of mild intermittent hypoxia exposure on the abdominal subcutaneous adipose tissue proteome in overweight and obese men: A first-in-human randomized, single-blind, cross-over study

Project description:Adipose tissue (AT) oxygen tension (pO2) has been implicated in AT dysfunction and metabolic perturbations in both rodents and humans. Compelling evidence suggests that hypoxia exposure alters metabolism, at least partly through effects on AT. However, it remains to be elucidated whether mild intermittent hypoxia (MIH) exposure impacts the AT proteome. We performed a randomized, single-blind, cross-over study to investigate the effects of seven consecutive days of MIH (FiO2 15%, 3x2h/d) compared to normoxia (FiO2 21%) exposure, on the AT proteome in overweight/obese men. AT insulin sensitivity was determined by a two-step hyperinsulinemic-euglycemic clamp, and abdominal subcutaneous AT biopsies were collected (n=11) under normoxic, fasting conditions following both exposure regimens. AT proteins were isolated and quantified using liquid chromatography-mass spectrometry. After correction for blood contamination, 1022 AT protein IDs were identified, of which 123 were differentially expressed following MIH (p < 0.05). These proteins were involved in redox systems, cell-adhesion, actin cytoskeleton organization, extracellular matrix composition and energy metabolism. We demonstrate for the first time that MIH exposure impacts the AT proteome. Moreover, differential AT TMOD3 expression is related to changes in AT insulin sensitivity, thereby linking MIH-induced effects on the AT proteome to metabolic changes in overweight/obese humans.

2022-02-16 | PXD029213 | Pride

Transcriptomic responses to hypoxia in endometrial stromal fibroblasts and decidual stromal cells

Project description:We sequenced mRNA from hypoxia treated endometrial stromal fibroblasts and decidual stromal cells in order to better understand endometrial hypoxia responses

2019-01-01 | GSE111570 | GEO

RNAseq for subpopulations of adipose tissue stromal cells from mouse inguinal adipose tissue

Project description:RNAseq for subpopulations of adipose tissue stromal cells from mouse inguinal adipose tissue

| PRJEB41572 | ENA

Adipose tissue exposed to cold

Project description:Cold triggers VEGF dependent but hypoxia independent angiogenesis in adipose tissues and anti-VEGF agents modulate adipose metabolism The molecular mechanisms of angiogenesis in relation to adipose tissue metabolism remain poorly understood. Here we show that exposure of mice to cold led to conversion of white adipose tissue (WAT) to brown-like adipose tissue, accompanying the switch of an active angiogenic phenotype. Gene expression profile analysis showed VEGF was upregulated via most likely hypoxia-independent PGC-1 transcriptional activation. Intriguingly, VEGFR2 blockage abolished the cold-induced angiogenesis, significantly impaired nonshivering thermogenesis capacity, and markedly reduced adipose metabolism. Unexpectedly, VEGFR1 blockage resulted in opposite effects by increasing adipose vascularity and metabolism. These findings demonstrate that VEGFR2 and VEGFR1 mediate polarized activities in modulating adipose angiogenesis and metabolism. Taken together, our findings have conceptual implications in applying angiogenesis modulators for the treatment of obesity and metabolic disorders. Keywords: Time course

2009-02-01 | GSE13432 | GEO

Transcriptome profiling of brown adipose tissue during cold exposure

Project description:We applied a deep-sequencing based method – digital gene expression profiling (DGEP), to investigate gene expression in interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) in acute cold exposure Examination of gene expression level in 3 different adipose tissues in 3 time points, day0, day2 and day4 in cold exposure.

2014-12-19 | E-GEOD-63031 | biostudies-arrayexpress

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