Project description:Proteomic samples were harvested from different life cycle stages of the invasive pest Lycorma Delicatula, or the Spotted Lanternfly. Adults were captured in the wild while egg and early instar samples were obtained by hatching insects in captivity. Samples were homogenized by bead disruption after percussive homogenization of samples frozen at -80C. Homogenization in 1x S-trap lysis buffer (Protifi, Long Island, NY) was performed in 30s pulses until homogenization appeared complete by visual inspection. The resulting lysate was reduced in DTT and alkylated with iodoacetamide prior to S-Trap mini digestion following vendor protocols. The resulting peptides were analyzed by diaPASEF based proteomics using the default workflow for "short gradient diaPASEF" in TimsControl 4.0. Raw files were processed with a 6 frame translation of the L. delicatula genome sequence and annotated with EggNog Mapper V2 with peptide match and quantification performed with SpectroNaut 18. Please see the associated metadata excel sheet for sample identities.
