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Molecular Genetics Of Heterotaxy And Related Congenital Heart Defects

ABSTRACT: Molecular Genetics Of Heterotaxy And Related Congenital Heart Defects

PROVIDER: PRJNA540544 | ENA |

REPOSITORIES: ENA

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Molecular Genetics Of Heterotaxy And Related Congenital Heart Defects

Project description:Molecular Genetics Of Heterotaxy And Related Congenital Heart Defects

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Copy number variants analysis of heterotaxy syndrome patients with congenital heart defects

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Project description:Heterotaxy is a disorder characterized by severe congenital heart defects (CHDs) and abnormal left-right patterning in other thoracic or abdominal organs. Clinical and research-based genetic testing has previously focused on evaluation of coding variants to identify causes of CHDs, leaving non-coding causes of CHDs largely unknown. Variants in the transcription factor Zinc finger of the cerebellum 3 (ZIC3) cause X-linked heterotaxy. We identified an X-linked heterotaxy pedigree without a coding variant in ZIC3. Whole genome sequencing revealed a deep intronic variant (ZIC3 c.1224+3286A>G) predicted to alter RNA splicing. An in vitro minigene splicing assay confirmed the variant acts as a cryptic splice acceptor. CRISPR/Cas9 served to introduce the ZIC3 c.1224+3286A>G variant into human embryonic stem cells demonstrating pseudoexon inclusion caused by the variant. Surprisingly, Sanger sequencing of the resulting ZIC3 c.1224+3286A>G amplicons revealed several isoforms, many of which by-pass the normal coding sequence of the third exon of ZIC3, causing a disruption of a DNA binding domain and a nuclear localization signal. Short- and long-read mRNA sequencing confirmed these initial results and identified additional splicing patterns. Assessment of four isoforms determined abnormal functions in vitro and in vivo while treatment with a splice-blocking morpholino partially rescued ZIC3. These results demonstrate that pseudoexon inclusion in ZIC3 can cause heterotaxy and provide functional validation of non-coding disease causation. Our results suggest the importance of non-coding variants in heterotaxy and the need for improved methods to identify and classify non-coding variation that may contribute to CHDs.

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Molecular Genetics of Heterotaxy and Related Congenital Heart Defects

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