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Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

ABSTRACT: Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

PROVIDER: PRJNA540604 | ENA |

REPOSITORIES: ENA

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Gene expression profiling identifies potential biomarkers for colorectal cancer using NanoString nCounter assay

Project description:The purpose of this study was to identify the differentially expressed genes and new potential predictive and prognostic molecular biomarkers for CRC. We identified 101 genes that were upregulated and 76 genes that were downregulated in metastatic tumors. After validation, a reduced set of 27 genes revealed evident correlation with CRC in the tumor tissue. Several genes in the pan-cancer panel showed significant differential expression, which were more universal than others in the CRC tissue. Since some of them have not been reported as being directly related to CRC yet, future mechanism studies can be designed based on this study.Our study demonstrated NanoString nCounter assay could serve as an alternative approach for gene expression analysis and identified several unreported differently expressed genes in CRC patients, which may provide some important clues for more in-depth study of CRC and may serve as potential predictive molecular biomarkers for clinical diagnosis application as well.

2019-05-05 | GSE130557 | GEO

Nanostring nCounter human miRNA assay (v1) of eosinophilic esophagitis

Project description:Nanostring nCounter Human miRNA assay (v1) of esophageal mucosal biopsies from children with eosinophilic esophagitis and controls Individual esophageal mucosal biopsies from children with eosinoniphilic esophagitis and controls were analysed for detection of microRNA

2014-09-01 | E-GEOD-55794 | biostudies-arrayexpress

Nanostring nCounter human miRNA assay (v1) of eosinophilic esophagitis

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Concordance of PAM50 molecular subtyping between oligonucleotide microarray and NanoString nCounter assay for Taiwanese breast cancer

Project description:Five molecular subtypes have been identified according to intrinsic genes transcription. Microarrays have been adopted for molecular subtyping in addition to the original NanoString nCounter assay. This study aimed to evaluate subtyping consistency among Taiwanese breast cancers. Our study showed that fundamental discrepancy exists between distinct gene expression measuring approaches, and cross platform equivalence should not be overemphasized with too much extrapolation.

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nCounter analysis of mRNAs associated with GBM tissue using a Nanostring custom assay

Project description:nCounter analysis of mRNAs associated with GBM tissue using a Nanostring custom assay

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miRQC data for NanoString nCounter miRNA Expression Assays.

Project description:nCounter miRNA Expression Assay data for all profiled miRQC samples prepared by the miRQC Consortium. In this study, we systematically compared 12 commercially available miRNA expression platforms by measuring an identical set of standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples, and synthetic spikes from miRNA family members with varying homology. We developed novel and robust quality metrics to objectively assess platform performance of very different technologies such as small RNA sequencing, RT-qPCR and (microarray) hybridization. We assessed reproducibility, sensitivity, accuracy, specificity, and concordance of differential expression. miRQC Consortium multi-platform comparision. *This represents the NanoString component only

2013-12-30 | E-GEOD-49600 | biostudies-arrayexpress

nCounter NanoString Human Immunology V2 panel

Project description:nCounter NanoString Human Immunology V2 panel

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Nanostring ncounter NHP Immunology V2 panel

Project description:Nanostring ncounter NHP Immunology V2 panel

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NanoString nCounter Human HCC expression analysis

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Nanostring nCounter expression analysis of sea lamprey embryos and adult testes (Petromyzon marinus)

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2016-10-21 | E-GEOD-76064 | biostudies-arrayexpress

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