Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells

ABSTRACT: Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells

PROVIDER: PRJNA540788 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9002760_1.fastq.gz	Fastqsanger.gz
	SRR9002760_2.fastq.gz	Fastqsanger.gz
	SRR9002761_1.fastq.gz	Fastqsanger.gz
	SRR9002761_2.fastq.gz	Fastqsanger.gz
	SRR9002762_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells

Project description:NOD mice were treated with PBS or OX40L and Jagged-1 proteins once a week for three weeks. A week after final treatment CD4+CD25- Teff cells and CD4+CD25hi Treg cells were sorted by FACS. Genomic DNA isolated from sorted cells and differences in DNA methylation pattern in these cell types between PBS and OX40L-Jagged-1 treated mice was analyzed by whole genome bi-sulfite sequencing.

2019-05-03 | GSE130617 | GEO

Differential gene expression microarray analysis of Tregs and Teff cells expanded by TCR-dependent Vs independent methods

Project description:CD4+ cells from Foxp3.eGFP mice containing Foxp3- Teff and Foxp3+ Treg cells were treated with anti-CD3/CD28 monoclonal antibodies or soluble OX40L and JAG1 for 3 days to induce TCR-dependent vs TCR-independent Treg proliferation. Untreated fresh CD4+ T-cells used as control. Post treatment T-cell proliferation was confirmed by Cell Trace violet dilution and Foxp3+ (Treg) and Foxp3-(Teff) were sorted. Differential gene expression profiling between Tregs and Teff cells among control, anti-CD3/CD28 and OX40L-JAG1 treated cultured was performed using affymetrix mouse gene 2.0 ST micro array. We used microarrays to detail the global programme of gene expression underlying Treg proliferation and identified distinct classes of up-regulated genes during this process under different methods of expansion.

2019-08-29 | GSE136582 | GEO

Differential gene expression profiling of conventional (Tconv), regulatory (Treg), follicular (TFH) helper and follicular regulatory (TFR) T-cells from control vs OX40L-Jagged-1 treated NZBWF1/j mice

Project description:Differential gene expression profiling of conventional (Tconv), regulatory (Treg), follicular (TFH) helper and follicular regulatory (TFR) T-cells from control vs OX40L-Jagged-1 treated NZBWF1/j mice

2022-03-17 | GSE181433 | GEO

OX40L protects against inflammation-driven fibrosis

Project description:Fibrosis is a leading cause of deaths in industrialized countries and has no effective therapy. We demonstrated that blockade of OX40L prevented inflammation-driven fibrosis affecting the skin and the lungs and promotes regression of established dermal fibrosis in different murine models. To characterize the pathways involved in the protection of skin fibrosis and affected by OX40L blocking, we used microarrays and identified distinct genes differentially expressed between ox40l+/+ and ox40l-/- in the bleomycin-induced dermal fibrosis mouse model. Total RNA were extracted from lesional skin samples of 3 ox40l+/+ and 4 ox40l-/- male mice aged 9 weeks treated with bleomycin for 3 weeks, and were hybridized on Affymetrix microarrays.

2016-08-09 | E-GEOD-73705 | biostudies-arrayexpress

OX40L protects against inflammation-driven fibrosis

2016-08-09 | GSE73705 | GEO

Differential gene expression microarray analysis of Tregs and Teff cells expanded by TCR-dependent Vs independent methods

Project description:Differential gene expression microarray analysis of Tregs and Teff cells expanded by TCR-dependent Vs independent methods

| PRJNA562731 | ENA

Exosomal OX40L elicits positive T cell co-stimulation and promotes anti-tumor immunity through engagement of cytotoxic T lymphocytes

Project description:Exosomes play a role in immune regulation associated with cancer and other diseases. We generated exosomes that harbor the ligand of the co-stimulatory OX40 pathway, and demonstrated abundant levels of OX40L on the surface of exosomes, without inducing morphological and molecular alterations. Functionally, exosomal OX40L efficiently elicits positive co-stimulation of both human and murine T cells. The systemic administration of exosomal OX40L promotes anti-tumor immunity in an orthotopic melanoma mouse model, and the depletion of T cell subsets revealed that exosomal OX40L acts through CD8+ T cells. Mechanistically, exosomal OX40L enhances the proportion and the levels of cytotoxic markers in tumor-infiltrating CD8+ T lymphocytes. Taken together, using the OX40 pathway as proxy, we provide proof-of-principle evidence that the presence of positive co-stimulatory molecules on exosomes can functionally engage T cells to elicit anti-tumor immunity, paving the way to understand the biology of exosome-mediate T cell signaling and for further developing exosome-based reagents that engage T cell co-stimulation.

2025-02-10 | PXD031404 | Pride

Comparative analysis of mouse CD8+ Teff cells infiltrating distinct organ targets of aGVHD by gene expression profiling

Project description:This study seeks a better understanding of the functional differences emerging between mouse CD8+ T effector (Teff) cells infiltrating three distinct mucosal environments; the small intestine, the lung and the liver. CD8+ Teff cells immigrating in the target organs were generated using a murine experimental acute graft versus host disease (aGVHD) model. For this purpose, congenially labeled CD45.1 OT-I CD8+ Teff cells were transplanted to and activated in a lethally irradiated allogeneic CD45.2 host constitutively expressing their cognate antigen, the chicken ovalbumin protein. CD8+ Teff cells were retrieved from their respective organ environments by automated organ disintegration and MACS sorting, and compared with each other by whole genome gene expression profiling. Peripheral blood derived circulating CD8+ Teff cells were also analyzed, serving as circulating peripheral T effector cell controls

2019-01-04 | GSE65043 | GEO

A STAT3 inhibitor ameliorates CNS autoimmunity by restoring Teff:Treg balance.

Project description:Reestablishing an appropriate balance between T effector cells (Teff) and Tregs is essential for correcting autoimmunity. Multiple sclerosis (MS) is an immune-mediated chronic CNS disease characterized by neuroinflammation, demyelination, and neuronal degeneration, in which the Teff:Treg balance is skewed toward pathogenic Teffs Th1 and Th17 cells. STAT3 is a key regulator of Teff:Treg balance. Using the structure-based design, we have developed a potentially novel small-molecule prodrug LLL12b that specifically inhibits STAT3 and suppresses Th17 differentiation and expansion. Moreover, LLL12b regulates the fate decision between Th17 and Tregs in an inflammatory environment, shifting Th17:Treg balance toward Tregs and favoring the resolution of inflammation. Therapeutic administration of LLL12b after disease onset significantly suppresses disease progression in adoptively transferred, chronic, and relapsing-remitting experimental autoimmune encephalomyelitis. Disease relapses were also significantly suppressed by LLL12b given during the remission phase. Additionally, LLL12b shifts Th17:Treg balance of CD4+ T cells from MS patients toward Tregs and increases Teff sensitivity to Treg-mediated suppression. These data suggest that selective inhibition of STAT3 by the small molecule LLL12b recalibrates the effector and regulatory arms of CD4+ T responses, representing a potentially clinically translatable therapeutic strategy for MS.

| S-EPMC7934926 | biostudies-literature

ATAC-Seq examining the TFH and TEFF primed in Wild-type and IL6 KO mice

Project description:The goal of this study was to determine if there were differences in chromatin accessiblity between TFH and TEFF developing in Wild-type and IL6 KO mice

2019-07-17 | GSE124588 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data