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Bos taurus breed:Holstein

ABSTRACT: Raw sequence reads of whole genome DNA methylation sequencing of bovine blastocyst derived from in vivo, fresh and vitrified oocytes after IVF

PROVIDER: PRJNA541282 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9034690_1.fastq.gz	Fastqsanger.gz
	SRR9034690_2.fastq.gz	Fastqsanger.gz
	SRR9034691_1.fastq.gz	Fastqsanger.gz
	SRR9034691_2.fastq.gz	Fastqsanger.gz
	SRR9034692_1.fastq.gz	Fastqsanger.gz

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Project description:In vitro maturation (IVM) of the oocytes is a routine method in bovine embryo production. The competence of bovine oocytes to develop into embryo after IVM and in vitro fertilization (IVF) is lower as compared to in vivo preovulatory oocytes. Cumulus cells (CC) that enclose an oocyte are involved in the acquisition of oocyte quality during maturation. Using transcriptomic approach we compared cumulus cells gene expression during IVM with that in vivo preovulatory period. Global transcriptional profiling was performed using cumulus cells collected from mature bovine oocytes (metaphase-II stage) after maturation performed either in vivo or in vitro. In vivo matured cumulus cells were collected from ovulatory follicles of Montbeliard adult cows by ovum pick-up in vivo (OPU, n=4). In vitro matured cumulus cells were recovered from the oocytes after 22h of in vitro culture of cumulus-oocyte complexes (50 COC per experiment) from 2-6 mm ovarian follicles of adult cows (MIV, n=4). Gene expression analysis was carried out between in vivo and in vitro matured cumulus representing a total of 8 slides (dye swap protocol)

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Project description:scWGBS bovine MII oocyte derived from vitrified GV oocyte sample 2

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Project description:scWGBS bovine MII oocyte derived from vitrified GV oocyte sample 3

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