Project description:1-day-old seedlings of Col-0 and vil1-1 were performed RNA-Seq to identify differentially expressed genes caused by VIL1 mutation in Arabidopsis
Project description:Cytokinin (CK) has been known as a cell proliferation activator for a long time in plants; however, its regulatory mechanism in cell division and expansion still remains unclear. Here we revealed that a bHLH transcription factor, CKG (CYTOKININ-RESPONSIVE GROWTH REGULATOR) mediates CK-dependent regulation of cell expansion and cell cycle. Overexpression of CKG increased cell size in a ploidy-independent manner and promoted S-phase entry of cell-cycle especially at early developmental stage, and pleiotropically enhanced organ growth from embryogenesis to reproductive stage, particularly cotyledons. In contrast, ckg loss-of-function mutant exhibited smaller cotyledons. CKG regulates the expression of genes mainly involved in regulation of cell cycle and macromolecule synthesis. We propose that CKG provides a regulatory module of cell cycle and organ growth in CK response.
Project description:Transcriptional profiling of Arabidopsis thaliana cotyledons comparing ecotype Col-0 (Control) with lea13 T-DNA line to elucidate the response mechanism to drought stress conditions that rely on LEA protein function.
Project description:To analyze the molecular mechanism behind the relationship between Arabidopsis leaf maturation and de novo root regeneration, we carried out an RNA-seq analysis using detached first-pair rosette leaves before culturing (time 0) and 1 d after culturing (DAC) from 9-, 12- and 15-d-old Col-0 seedlings. We first analyzed gene expression levels in the leaves before detachment (at time 0) from the three developmental states. Changes in gene expression could be grouped into six clusters. Many genes were upregulated or downregulated during leaf maturation. Next, we analyzed gene expression levels in the leaf explants from 9- and 15-d-old seedlings at 1 DAC compared with gene expression levels at time 0. By comparing up- or downregulated genes (1 DAC vs time 0) between leaves from 9- and 15-d-old seedlings, we found that many of the genes were particularly up- or downregulated only in immature leaves or only in mature leaves after 1 d of culturing.
Project description:Transcriptome-wide analysis of gene expression using detached first-pair rosette leaves before culture (time 0) and 1 day after culture (DAC) from 9-day-old, 12-day-old and 15-day-old Col-0 seedlings
Project description:We determined the transcriptomes of hda9-1, pif4-2 and wild type Col-0 seedlings of 2 day old and 7 day old, at control and high temperature conditions (22oC vs 27oC), in short day (8h) photoperiod
Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the total RNA sequencing.
Project description:To reveal the molecular mechanism during de novo root regeneration from Arabidopsis leaf explants cultured on B5 medium without exogenous hormones, we carried out an RNA-seq experiment using detached leaf explants with partial petiole before culture (i.e. time 0) and 2 d after culturing (DAC) from12-d-old Col-0 seedlings. Gene expression of the wounded region (including the partial petiole and some surrounding tissues), which comprises regeneration-competent cells, was analyzed.