Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic dissection of gene silencing mediated by H3K27 methylation

ABSTRACT: Genetic dissection of gene silencing mediated by H3K27 methylation

PROVIDER: PRJNA542356 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9041320.fastq.gz	Fastqsanger.gz
	SRR9041330.fastq.gz	Fastqsanger.gz
	SRR9041340.fastq.gz	Fastqsanger.gz
	SRR9041350.fastq.gz	Fastqsanger.gz
	SRR9041360.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 68

Similar Datasets

Genetic dissection of gene silencing mediated by H3K27 methylation

Project description:We are intereted in changes in transcriptome in putative polycomb mutants generated by exposure to UV light. We included polycomb mutants made by reverse genetics and other WT strains with different genetic backgrounds used as parents for bulk segregant analysis.

2020-01-01 | GSE131036 | GEO

Gene expression and H3K27 tri-methylation analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants

Project description:This SuperSeries is composed of the following subset Series: GSE24464: Expression analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants GSE24474: H3K27 tri-methylation analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants Refer to individual Series

2011-04-20 | E-GEOD-24507 | biostudies-arrayexpress

Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast

Project description:Constitutive domains of repressive heterochromatin are maintained within the fission yeast genome through self-reinforcing mechanisms involving histone methylation and small RNAs. Non-coding RNAs generated from heterochromatic regions are processed into small RNAs by the RNA interference pathway, and are subject to silencing through both transcriptional and post-transcriptional mechanisms. While the pathways involved in maintenance of the repressive heterochromatin state are reasonably well understood, less is known about the requirements for its establishment. Here we describe a novel role for the post-transcriptional regulatory factor Mkt1 in establishment of heterochromatin at pericentromeres in fission yeast. Loss of Mkt1 does not affect maintenance of existing heterochromatin, but does affect its recovery following depletion, as well as de novo establishment of heterochromatin on a mini-chromosome. Pathway dissection revealed that Mkt1 is required for RNAi-mediated post-transcriptional silencing, downstream of small RNA production. Mkt1 physically associates with pericentromeric transcripts, and is additionally required for maintenance of silencing and heterochromatin at centromeres when transcriptional silencing is impaired. Our findings provide new insight into the mechanism of RNAi-mediated post-transcriptional silencing in fission yeast, and unveil an important role for post-transcriptional silencing in establishment of heterochromatin that is dispensable when full transcriptional silencing is imposed.

2019-11-28 | PXD015484 | Pride

Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation

Project description:Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation

| PRJNA634024 | ENA

H3K36 methylation antagonizes PRC2-mediated H3K27 methylation.

Project description:H3K27 methylation mediated by the histone methyltransferase complex PRC2 is critical for transcriptional regulation, Polycomb silencing, Drosophila segmentation, mammalian X chromosome inactivation, and cancer. PRC2-mediated H3K27 methylation can spread along the chromatin and propagate the repressive chromatin environment; thus, chromatin components that antagonize the activity of PRC2 are important for restraining Polycomb silencing. Here we report that in HeLa cells, H3 histones unmethylated at Lys-36 are mostly methylated at Lys-27, with the exception of newly synthesized H3. In addition, K27me3 rarely co-exists with K36me2 or K36me3 on the same histone H3 polypeptide. Moreover, PRC2 activity is greatly inhibited on nucleosomal substrates with preinstalled H3K36 methylation. These findings collectively identify H3K36 methylation as a chromatin component that restricts the PRC2-mediated spread of H3K27 methylation. Finally, we provide evidence that the controversial histone lysine methyltransferase Ash1, a known Trithorax group protein that antagonizes Polycomb silencing in vivo, is an H3K36-specific dimethylase, not an H3K4 methylase, further supporting the role of H3K36 methylation in antagonizing PRC2-mediated H3K27 methylation.

| S-EPMC3048685 | biostudies-literature

Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [HiChIP]

Project description:Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [HiChIP]

| PRJNA634029 | ENA

Loss of H3K27 monomethylation leads to DNA-methylation-independent release of gene silencing

Project description:Constitutive heterochromatin in Arabidopsis thaliana is marked by repressive chromatin modifications including DNA methylation, histone 3 dimethylation at lysine 9 (H3K9me2), and monomethylation at lysine 27 (H3K27me1). The enzymes catalyzing DNA methylation and H3K9me2 have been identified and mutations in these proteins lead to the reactivation of silenced heterochromatic elements. The enzymes responsible for heterochromatic H3K27me1, in contrast, remain unknown. Here we show that the divergent SET-domain proteins ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) and ATXR6 exhibit H3K27 monomethyltransferase activity and double mutants have reduced H3K27me1 in vivo and show partial heterochromatin decondensation. atxr5 atxr6 plants also show transcriptional activation of repressed heterochromatic elements. Interestingly, H3K9me2 and DNA methylation are unaffected in the double mutant. These results indicate that ATXR5 and ATXR6 form a novel class of H3K27 methyltransferases and that H3K27me1 represents a new pathway required for transcriptional repression in Arabidopsis. Comparison of methylation in wild type and ATXR6/ATXR6 mutants

2009-05-31 | E-GEOD-16071 | biostudies-arrayexpress

Analysis of genes bound by ULK3 and of H3K27 histone modification plus/minus ULK3 silencing in SCC13 cells

Project description:We report high-throughput profiling of genes bound by ULK3 and of H3K27 histone modification plus/minus ULK3 silencing in squamous cell carcinoma SCC13 cells .

2022-10-10 | GSE183933 | GEO

Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [4C-seq]

Project description:Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [4C-seq]

| PRJNA634027 | ENA

Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [Cut&Tag]

Project description:Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation [Cut&Tag]

| PRJNA634028 | ENA