Project description:The understanding of host responses to Tuber colonization would facilitate the exploration of symbiotic interactions and contribute to truffle artificial cultivation. T. indicum and T. panzhihuanense were selected to colonize Pinus armandii and Carya illinoinensis for a two-and-a-half-year symbiosis experiment in this study. The molecular response of host leaf to Tuber symbiosis was analyzed using RNA-seq. T. indicum and T. panzhihuanense both triggered the defense-related regulation in host leaf, like secondary metabolism, cell wall biogenesis, plant hormone signal transduction and plant-pathogen interaction, with different patterns in P. armandii and C. illinoinensis.