Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
Project description:Comprehensive RNA sequencing was performed on a laboratory colony of B. dorsalis with a focus on attempting to capture as many genes in the sequencing from throughout the entire developmental life history. De novo assembly and analysis of the resulting sequence One sample each for the egg, larvae, pupae, adult male, adult female and mated female life stages was sequenced.
Project description:We constructed three RNA libraries from embryos at 0-1, 2-4 and 5-8 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. Using an Illumina HiSeq 2500 platform, we mapped more than 83, 85 and 98 million sequence reads from 0-1, 2-4 and 5-8 hour embryos respectively, and identified a total of 13,489 unigenes. 1683, 3201 and 3134 unigenes showed differential expression between the 0-1 and 2-4 hour embryos, the 0-1 and 5-8 hour embryos, and the 2-4 and 5-8 hour embryos respectively.
