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ABSTRACT: Characterization of the Daphnia magna microbiota

PROVIDER: PRJNA543317 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9072116_1.fastq.gz	Fastqsanger.gz
	SRR9072116_2.fastq.gz	Fastqsanger.gz
	SRR9072117_1.fastq.gz	Fastqsanger.gz
	SRR9072117_2.fastq.gz	Fastqsanger.gz
	SRR9072118_1.fastq.gz	Fastqsanger.gz

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Long-term exposure of Daphnia magna to polystyrene microplastics induces changes in their proteome.

Project description:In the past years, the research focus on the effects of microplastics (MP) on aquatic organisms extended from marine systems towards freshwater systems. An important freshwater model organism in the MP field is the cladoceran Daphnia, which plays a central role in lacustrine ecosystems and has been established as a test organism in ecotoxicology. To investigate the effects of MP on Daphnia magna, we performed a chronic exposure experiment with polystyrene MP under strictly standardized conditions. Chronic exposure of D. magna to PS microparticles led to a significant reduction in body length and number of offspring. To shed light on underlying molecular mechanisms induced by microplastic ingestion in D. magna, we assessed the effects of PS-MP at the proteomic level.

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Whole organism transcriptomic analysis provides mechanistic insight into the acute toxicity of emamectin benzoate in Daphnia magna

Project description:This experiment was conducted to study the short-term (12h) transcriptional responses in Daphnia magna after exposure to the anti-sea lice chemical emamectin benzoate (EMB). The microarray results were further vefiried using qPCR. The gene exression responses were linked to adverse effects after 48h exposure, in order to supply knowledge for environmental hazard assessment of this chemical in non-target crustaceans. Neonatal (<24h) Daphnia magna were exposed to 7.8-2000 pM waterborne emamectin benzoate for 12h. Microarray analysis was performed using pooled whole-organism D. magna (8 individuals) and 4 biological replicates were analyzed for each treatment group.

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