Pisum sativum
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ABSTRACT:
PROVIDER: PRJNA543764 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18323754.fastq.gz | Fastqsanger.gz | |||
| SRR18323755.fastq.gz | Fastqsanger.gz | |||
| SRR18323756.fastq.gz | Fastqsanger.gz | |||
| SRR18323757.fastq.gz | Fastqsanger.gz | |||
| SRR18323758.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Seeds of Pisum sativum L. cv Alaska Early (purchased from the ‘Abundant Life Seed Foundation’, Port Townsend, USA) were equilibrated for approximately 5 weeks in tightly sealed boxes over 300 g-1 non-saturated LiCl (Hay et al. 2008), producing 60% relative humidity, in a temperature-controlled room (20 ± 1 ºC) until their water potential was stable at 12 % MC. Relative humidity was recorded with a Rotronic AWVC-D10 Hygropalm. These equilibrated seeds are referred to as 0 day ‘non-aged controls’. Following equilibration, accelerated ageing was carried out by placing seeds in sealed bottles at 50 °C until viability loss, thereafter referred to as ‘ageing’. Samples were taken at intervals after 8, 12, and 15 days of ageing. At each interval, germination tests were performed on 1 % water agar at 25 °C under warm white fluorescent light (15 micromol m-2 s-1) at a day / night cycle (8/16h). Germination was defined as radical emergence by at least 2 mm. To summarize, we have performed a transcriptional profiling of Pisum sativum seeds comparing 0 day ‘non-aged controls’ with 8, 12 and 15 days of artificially aged seeds.
2010-10-22 | GSE24864 | GEO
Project description:Seeds of Pisum sativum L. cv Alaska Early (purchased from the âAbundant Life Seed Foundationâ, Port Townsend, USA) were equilibrated for approximately 5 weeks in tightly sealed boxes over 300 g-1 non-saturated LiCl (Hay et al. 2008), producing 60% relative humidity, in a temperature-controlled room (20 ± 1 ºC) until their water potential was stable at 12 % MC. Relative humidity was recorded with a Rotronic AWVC-D10 Hygropalm. These equilibrated seeds are referred to as 0 day ânon-aged controlsâ. Following equilibration, accelerated ageing was carried out by placing seeds in sealed bottles at 50 °C until viability loss, thereafter referred to as âageingâ. Samples were taken at intervals after 8, 12, and 15 days of ageing. At each interval, germination tests were performed on 1 % water agar at 25 °C under warm white fluorescent light (15 micromol m-2 s-1) at a day / night cycle (8/16h). Germination was defined as radical emergence by at least 2 mm. To summarize, we have performed a transcriptional profiling of Pisum sativum seeds comparing 0 day ânon-aged controlsâ with 8, 12 and 15 days of artificially aged seeds. Four-condition experiment, 0 days, 8 days, 12 days and 15 days. Biological replicates: 3 for each comparison. Each biological replicate was independently grown and harvested.
2010-10-22 | E-GEOD-24864 | biostudies-arrayexpress
Project description:Pea (Pisum. sativum L.) is a traditional and important edible legume that can be sorted into grain pea and vegetable pea according to their harvested maturely or not. Vegetable pea by eating the fresh seed is becoming more and more popular in recent years. These two type peas display huge variations of the taste and nutrition, but how seed development and nutrition accumulation of grain pea and vegetable pea and their differences at the molecular level remains poorly understood. To understand the genes and gene networks regulate seed development in grain pea and vegetable pea, high throughput RNA-Seq and bioinformatics analysis were used to compare the transcriptomes of vegetable pea and grain pea developing seed. RNA-Seq generated 18.7 G raw data, which was then de novo assembled into 77,273 unigenes with a mean length of 930 bp. Functional annotation of the unigenes was carried out using the nr, Swiss-Prot, COG, GO and KEGG databases. There were 459 and 801 genes showing differentially expressed between vegetable pea and grain pea at early and late seed maturation phases, respectively. Sugar and starch metabolism related genes were dramatically activated during pea seed development. The up-regulated of starch biosynthesis genes could explain the increment of starch content in grain pea then vegetable pea; while up-regulation of sugar metabolism related genes in vegetable pea then grain pea should participate in sugar accumulation and associated with the increase in sweetness of vegetable pea then grain pea. Furthermore, transcription factors were implicated in the seed development regulation in grain pea and vegetable pea. Thus, our results constitute a foundation in support of future efforts for understanding the underlying mechanism that control pea seed development and also serve as a valuable resource for improved pea breeding.
2017-02-09 | GSE72573 | GEO
Project description:During seed growth, sugar and nitrogen compounds confer regulatory control on storage activities. Thus, seed storage production could be regulated by the supply of nutrients. In order to improve nitrogen flux into the embryo, transgenic pea lines were created where ADP-glucose pyrophosphorylase (AGP) from Pisum sativum has been repressed by RNAi approach in the seeds under control of the seed-specific LeB4 promotor (Bäumlein et al. Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within legumin box is essential for tissue-specific expression of a legumin gene. Plant J 1992 2: 233-239). The plastidial enzyme AGP catalyzes the reaction of glucose-1-phosphate and ATP to pyrophosphate and ADP-glucose, which is the substrate for starch synthase. The AGP activity and transcript levels were strongly decreased in three independent transgenic lines. Repression of AGP results in a wrinkled seed phenotype obviously due to transient accumulation of free sugars during maturation. Mature seeds have reduced starch content whereas the protein concentration is higher due to increased fractions of albumins and globulins. Repression of AGP interferes with storage protein metabolism and alters fluxes of nitrogen during seed growth. The influence of decreased AGP on altered gene expression in developing cotyledons was analysed using a 6k-Oligo-microarray. Ps6kOLI1 microarray hybridization were performed using three independent biological replicates of four developmental stages (20, 25, 30 and 35 DAP) from seeds of the transgenic line iAGP-3.
2008-09-01 | E-MEXP-1749 | biostudies-arrayexpress
Project description:In this study the effect of Rhizobium leguminosarum bv. viceae (Rlv) on seed proteome through shotgun proteomics analyses of healthy versus pathogen (Didymella pinodes) infected Pisum sativum plants was investigated.
2017-12-06 | PXD006617 | Pride
Project description:Pisum sativum (cv. Kudrnac) seeds were obtained from SEMO a.s., Smržice, Czechia. All plants used for the study grew in the experimental field plot at Veletiny, Czechia (49°02'N 17°33'E; 195 m a.s.l.). Sowing was done after the rainfall on June 1, 2022, and plants were grown in a natural environment without any additional treatment. After 35 days, the field was divided into three blocks (mock, SAt1, SAt2), each containing approxi-mately 100 plants. For five weeks, plants were weekly sprayed with 1.5 L water solution supplemented with 0.025% (v/v) Silwet L-77 (mock; K), or 100 µM salicylic acid and Silwet (final concentration as for the mock; SA-a). The third set was treated as the mock for two weeks, followed by four weeks with salicylic acid treatment (SA-b).
2023-03-17 | PXD040506 | Pride
Project description:The lower needs for chemical fertilisers make the Pisum Sativum well suited for sustainable agricultural systems. During the past decades, yield losses were mainly due to pathogenic infestation, with Ascochyta blight as the most severe disease. In this fungal disease complex Didymella pinodes, as the most aggressive pathogen, has been examined intensively. Despite the great number of varieties, no resistance was yet found and several strategies were developed to avoid infestation. In recent years enhanced resistance to pathogens due to previous infestation by symbiotic microorganisms was discovered in several plants. However, only little attention was drawn on the implications of P. Sativum´s symbionts (Rhizobia, Mycorrhiza) on the disease severity as well as the effects of the pathogen on the symbiotic interactions. Hence, here the implications of the pathogenic infestation on the tripartite symbiosis of P. Sativum on a molecular (proteomics, metabolomics) and physiological level, and moreover, the potential of the root symbionts to enhance resistance against Didymella pinodes are investigated.
2017-01-16 | PXD005701 | Pride
Project description:The lower needs for chemical fertilisers make the Pisum Sativum well suited for sustainable agricultural systems. During the past decades, yield losses were mainly due to pathogenic infestation, with Ascochyta blight as the most severe disease. In this fungal disease complex Didymella pinodes, as the most aggressive pathogen, has been examined intensively. Despite the great number of varieties, no resistance was yet found and several strategies were developed to avoid infestation. In recent years enhanced resistance to pathogens due to previous infestation by symbiotic microorganisms was discovered in several plants. However, only little attention was drawn on the implications of P. Sativum´s symbionts (Rhizobia, Mycorrhiza) on the disease severity as well as the effects of the pathogen on the symbiotic interactions. Hence, here the implications of the pathogenic infestation on the tripartite symbiosis of P. Sativum on a molecular (proteomics, metabolomics) and physiological level, and moreover, the potential of the root symbionts to enhance resistance against Didymella pinodes are investigated.
2017-01-16 | PXD005700 | Pride
Project description:RNASeq analysis of transgenic Arabidopsis lines overexpressing Pisum sativum SUN gene
| PRJEB83817 | ENA
Project description:Whole genome sequencing of 15 Pisum sativum and 1 Pisum fulvum for the GENOPEA project granted by ANR
| PRJNA285605 | ENA