Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TRPV1 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

ABSTRACT: TRPV1 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

PROVIDER: PRJNA544199 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9107980_1.fastq.gz	Fastqsanger.gz
	SRR9107980_2.fastq.gz	Fastqsanger.gz
	SRR9107990_1.fastq.gz	Fastqsanger.gz
	SRR9107990_2.fastq.gz	Fastqsanger.gz
	SRR9107971_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

TRPV1 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:Visceral sensory neurons encode distinct sensations from healthy organs and initiate pain states that are resistant to common analgesics. Transcriptome analysis is transforming our understanding of sensory neuron subtypes but has generally focused on somatic sensory neurons or the total population of neurons in which visceral neurons form the minority. Our aim was to define transcripts specifically expressed by sacral visceral sensory neurons, as a step towards understanding the unique biology of these neurons and potentially lead to identification of new analgesic targets for pelvic visceral pain. Our strategy was to identify genes differentially expressed between sacral dorsal root ganglia (DRG) that include somatic neurons and sacral visceral neurons, and adjacent lumbar DRG that comprise exclusively somatic sensory neurons. This was performed in male and female mice (adult and E18.5). By developing a method to restrict analyses to nociceptive Trpv1 neurons, a larger group of genes were detected as differentially expressed between spinal level. We identified many novel genes not previously been associated with pelvic visceral sensation or nociception. Limited sex differences were detected across the transcriptome of sensory ganglia, but more were revealed in sacral levels and especially in Trpv1 nociceptive neurons. These data will facilitate development of new tools to modify mature and developing sensory neurons and nociceptive pathways.

2020-03-02 | GSE131621 | GEO

E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

2020-03-02 | GSE131622 | GEO

Adult DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

2020-03-02 | GSE131619 | GEO

E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

| PRJNA544211 | ENA

Adult DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:Adult DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

| PRJNA544198 | ENA

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

Project description:We developed an approach to rapidly eliminate the subgroup of sensory neurons expressing the heat-gated cation channel TRPV1 from dissociated rat sensory ganglia using agonist treatment followed by density centrifugation. To identify transcripts predomintly expressed in TRPV1-positive neurons, we compared the transcriptome of all cells within sensory ganglia versus all cells without TRPV1 expressing neurons using RNA-Seq. Four replicate experiments with RNA from DRG neurons of one rat per experiment were performed. Dissociated neurons were split up in three parts, treated with solvent DMSO (0.1%), casaicin (10 µM), or RTX (100 nM) for 30 min followed by gradient centrifugation. RNA was extracted from the remaining pellet containing either all cells or all cells without TRPV1-positive neurons.

2015-05-12 | E-GEOD-59727 | biostudies-arrayexpress

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

2015-12-30 | E-MTAB-2789 | biostudies-arrayexpress

Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

| PRJNA544201 | ENA

The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics

2015-05-12 | GSE59727 | GEO

Profiling of visceral sensory neurons in dorsal root and nodose ganglia in BALB/c mice.

Project description:Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation. Keywords: Cell type comparison

2005-12-21 | GSE2917 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data