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DNA methylation during seed development in small-seeded chickpea

ABSTRACT: DNA methylation during seed development in small-seeded chickpea

PROVIDER: PRJNA544333 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9110444_1.fastq.gz	Fastqsanger.gz
	SRR9110444_2.fastq.gz	Fastqsanger.gz
	SRR9110445_1.fastq.gz	Fastqsanger.gz
	SRR9110445_2.fastq.gz	Fastqsanger.gz
	SRR9110446_1.fastq.gz	Fastqsanger.gz

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DNA methylation during seed development in small-seeded chickpea

Project description:In this study, we performed bisulphite of two stages of seed development in a small-seeded chickpea cultivar (Himchana 1) using Illumina platform. Paired-end reads were generated from 5 libraries. Data obtained in FASTQ files were pre-processed to remove adapters and low-quality reads. We identified methylation level at each cytosine residue covered in sequencing and differentially methylated regions (DMRs) between stages of seed development.

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Project description:In this study, we performed bisulphite of five stages of seed development in a large-seeded chickpea cultivar (JGK 3) using Illumina platform. Paired-end reads were generated from 11 libraries. Data obtained in FASTQ files were pre-processed to remove adapters and low-quality reads. We identified methylation level at each cytosine residue covered in sequencing and differentially methylated regions (DMRs) between stages of seed development.

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Project description:In this study, we aim to present a global view of transcriptome dynamics during seed development in a small-seeded chickpea (genotype Himchana 1). We generated about 1.5 billion high-quality reads from 24 libraries (leaf and seven seed developmental stages in three biological replicates) using Illumina high-throughput sequencing platform. We mapped the reads to the kabuli chickpea genome for estimation of their transcript abundance in different tissue samples. The transcriptome dynamics was studied by differential gene expression analyses between different samples/stages.

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