Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

ABSTRACT: A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

PROVIDER: PRJNA544466 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10509714_1.fastq.gz	Fastqsanger.gz
	SRR10509714_2.fastq.gz	Fastqsanger.gz
	SRR10509715_1.fastq.gz	Fastqsanger.gz
	SRR10509715_2.fastq.gz	Fastqsanger.gz
	SRR10509716_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

Project description:Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were differentiated into forebrain neural progenitors using a fully defined protocol with dual SMAD-inhibition. Cells were taken at four time points to monitor temporal changes in the transcriptome over the course of differentiation.

2020-01-14 | GSE131697 | GEO

Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

Project description:Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

| PRJNA1106827 | ENA

Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

Project description:This bulk RNAseq dataset is part of a dataset described in the manuscript titled "Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation". This dataset includes NPC derived neurons using a wild type iPSC line, and was used to validate a MS-117 maturation score which attempt to establish a socre system to assess neuronal maturation with iPSCs derived neurons.

2024-09-13 | GSE266359 | GEO

Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

Project description:NGN2-driven iPSC-to-neuron conversion is a popular method for human neurological disease modeling. In this study, we report a fully standardized approach for generating functional excitatory cortical neurons. This method utilizes clonal, targeted-engineered iPSC lines and employs fully defined reagents. We demonstrate that our protocol generates consistent excitatory cortical neurons at scale. Comprehensive temporal omics, electrophysiological, and morphological profilings indicate the continued progression of neuronal maturation for at least 150 days. Deep quantitative characterizations through transcriptomic, imaging, and functional assays reveal coordinated actions of multiple pathways driving neuronal maturation. The neurons express the majority of the key risk genes in Alzheimer's disease, Parkinson's disease, and autism, demonstrating the relevance of our protocol in modeling human neurological disorders. This well-defined method, profiling data, and functional characterization provide a solid and reliable framework for developing human in vitro neuronal models for disease modeling.

2023-10-18 | MSV000093150 | MassIVE

Post-transcriptional mechanisms distinguish human and chimpanzee forebrain progenitors

Project description:Post-transcriptional mechanisms distinguish human and chimpanzee forebrain progenitors

| PRJNA524484 | ENA

All-defined NGN2 neuron protocol reveals multifaceted signatures of neuronal maturation.

Project description:All-defined NGN2 neuron protocol reveals multifaceted signatures of neuronal maturation.

| PRJNA1022478 | ENA

Expression of constitutively active FOXO3 in murine forebrain leads to a loss of neural progenitors

Project description:We have generated transgenic mice with tetracycline-regulated conditional expression of a constitutively active allele of FoxO3 under the control of the forebrain-specific CaMKIIa promoter. In adult animals, there was a reduction of brain weight by 30% and an almost complete loss of the dorsal dentate gyrus with normal cortical layering. Interestingly, the adult mice showed motor hyperactivity and a selective loss of long-term memory with normal spatial learning. We observed enhanced apoptosis starting from day E10.5. Performing microarray expression analyses and Q-PCR validation with E12.5 forebrain RNA, we observed an over-representation of thalamic markers and an under-representation of cortical markers in transgenic as compared to control animals. Immunohistochemical data show a loss of progenitors in the lateral ventricles. Up-regulation of Pik3ip1 as a target gene of FoxO3 could be responsible for the observed increase in apoptosis. The obtained forebrain expression signature is reminiscent of a Pax6 knockdown phenotype showing that expression of this FoxO3 allele during development affected neural progenitor survival and overall brain development. Conclusion: Neural progenitors are vulnerable to constitutively active FoxO3-induced apoptosis. We sought to determine the transcriptional differences in forebrains from E12.5 mice expressing a constitutively active alleleof FoxO3 under the control of the forebrain-specific CaMKIIa promoter. To this end two time-pregnant dams were sacrificed 12 days after the vaginal plug was detected, and the embryos were prepared. Visual staging of the embryos confirmed their age. Genotyping and luciferase measurements were performed in order to assess presence and acitivity of the transgenes.

2012-09-13 | E-GEOD-21089 | biostudies-arrayexpress

All-defined NGN2 neuron protocol reveals multifaceted signatures of neuronal maturation [bulk_media]

Project description:All-defined NGN2 neuron protocol reveals multifaceted signatures of neuronal maturation [bulk_media]

| PRJNA1022480 | ENA

Expression of constitutively active FOXO3 in murine forebrain leads to a loss of neural progenitors

2012-09-13 | GSE21089 | GEO

Expansion of human colonic organoids from tissue biopsies in a fully defined hydrogel matrix

Project description:Expansion of human colonic organoids from tissue biopsies in a fully defined hydrogel matrix

| PRJNA613086 | ENA