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ABSTRACT: RNAseq of mock and Turnip mosaic virus (TuMV) infected plants

PROVIDER: PRJNA545306 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9151620_1.fastq.gz	Fastqsanger.gz
	SRR9151620_2.fastq.gz	Fastqsanger.gz
	SRR9151630_1.fastq.gz	Fastqsanger.gz
	SRR9151630_2.fastq.gz	Fastqsanger.gz
	SRR9151621_1.fastq.gz	Fastqsanger.gz

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Label-free quantitative proteomics on Nicotiana benthamiana plasmodesmal proteome following viral infection

Project description:We analyzed the PD-enriched fraction from Turnip mosaic virus (TuMV)-infected Nicotiana benthmiana (N. benthamiana) by using label-free quantitative proteomics of which 100 and 48 were significantly increased and decreased respectively when compared with mock plants.

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Project description:Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosaic virus (TuMV) infection on host translation initiation. Plants were grown in LC-1 soil in 12 hour days and infected by TuMV-GFP or mock-inoculated just prior to sending up bolts. Samples were taken from rosette leaves 10 days after inoculation. Only tissue fluorescing GFP was selected from the virus-infected samples. Similar tissue was sampled from mock-infected leaves. FLAG antibody was used to isolate RPL18-HF. The RNA co-immunoprecipitated with RPL18-HF is fully translation-initiated. This translation-initiated RNA, also referred to as polysomal RNA, was isolated and compared to total RNA under both mock and TuMV-infected states to find TuMV-induced changes in host translation initiation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jackson Moeller. The equivalent experiment is AT42 at PLEXdb.]

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Project description:WGBS-seq of mock and Turnip mosaic virus (TuMV) infected plants

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