Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ASBT

ABSTRACT: ASBT

PROVIDER: PRJNA546249 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1814120.fastq.gz	Fastqsanger.gz
	SRR1814120_1.fastq.gz	Fastqsanger.gz
	SRR1814120_2.fastq.gz	Fastqsanger.gz
	SRR1814121.fastq.gz	Fastqsanger.gz
	SRR1814121_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 37

Similar Datasets

Intestinal expression of ASBT determines the sclerosing cholangitis phenotype.

Project description:Purpose: Determine the impact of ASBT genotype on the onset and progression of liver disease, using RNA-sequencing to characterize the transcriptome of ASBT wildtype and knockout mice. We assess alterations at the gene and mechanistic-levels. Methods: mRNA profiles were generated in 45-day-old ASBT WT and KO mice (BALB/cJ background). Data were processed using the Tuxedo Pipeline, using the mm10 genome with annotations provided by Ensembl. Results: We identified 1406 differentially expressed transcripts between knockout and wildtype mice, with ontologies heavily weighted toward fibrotic and inflammatory processes, with immune cell infiltration. Conclusions: Knockout of ASBT induces increased liver injury through exposure to elevated bile acids.

2019-06-04 | GSE132168 | GEO

Effect of pharmacological inhibition of ASBT on bile composition and sclerosing cholangitis in mdr2 knockout mice

Project description:We report the effect of a potent pharmacological inhibition of ASBT in mdr2 -/- mice, compared to genetic and treatment controls using RNA-sequencing. Through quantification of mRNA in liver samples, we found significant upregulation of anti-inflammatory and anti-fibrotic gene signatures in mdr2-/- mice. Additionally, we report downregulation of pro-inflammatory genes invovled in leukocyte recruitment. Mdr2 knockout mice (female, 30 day old) were fed high fat-chow diet containing a potent inhibitor of ASBT for 14 days. Genotypic and dietary controls were included. RNA-sequencing was performed on liver samples taken from the caudate lobe.

2016-02-17 | E-GEOD-66231 | biostudies-arrayexpress

ASBT inhibition alleviates cholangiopathy and hepatic fibrosis development in Cyp2c70-/- mice

Project description:RNA-seq was used to compare the liver gene differentiation between WT and Cyp2c70-/- mice fed with chow and SC-435 containing diet. We aimed to characterize the cholestatic liver phenotype in the Cyp2c70 -/- mouse and test whether ASBT inhibition reduced injury.

2022-08-08 | GSE183251 | GEO

Escherichia coli strain:ASBT-1

Project description:Escherichia coli strain:ASBT-1 Genome sequencing and assembly

| PRJNA509104 | ENA

Effect of pharmacological inhibition of ASBT on bile composition and sclerosing cholangitis in mdr2 knockout mice

2016-02-17 | GSE66231 | GEO

Intestinal expression of ASBT determines the sclerosing cholangitis phenotype.

Project description:Intestinal expression of ASBT determines the sclerosing cholangitis phenotype.

| PRJNA546456 | ENA

ASBT inhibition alleviates cholangiopathy and hepatic fibrosis development in Cyp2c70-/- mice

Project description:ASBT inhibition alleviates cholangiopathy and hepatic fibrosis development in Cyp2c70-/- mice

| PRJNA759789 | ENA

ASBT

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-04 | GSE132170 | GEO

PRM targeted mass spectrometry reveals the phosphorylation regulation of human bile acid transporter ASBT

Project description:The human apical bile acid transporter (hASBT) in the distal ileum reabsorbs bile acids and is responsible for transporting approximately 95 % of bile acids back to liver for recycling in the enterohepatic pathway. ASBT activity has been linked to multiple disease states, including Crohn’s disease, hypercholesterolemia, cholestasis, and type 2 diabetes. ASBT activity is regulated at the post-translational level by glycosylation, ubiquitination, and S-acylation, which control its translocation to the cell surface and protein stability. Although biochemical studies have suggested that phosphorylation of serine, threonine, and tyrosine may play a role in the regulation of ASBT function, no study has determined where phosphorylation of ASBT occurs and how its phosphorylation level correlates with its activity. In this study, we developed a workflow using parallel reaction monitoring (PRM) targeted mass spectrometry to determine and quantify the stoichiometry of ASBT phosphorylation in the presence of various kinase inhibitors and activators. Our findings provide the first evidence of ASBT phosphorylation at multiple sites (Thr330, Ser334, and Ser 335), with Ser 335 being the most abundant phosphosite.Futhermore, we demonstrate that the phosphorylation level of Serine 335 coincides with the bile acid uptake activity of ASBT. Finally, we discovered that PKCalpha, but not other PKC isoforms, regulates ASBT phosphorylation at Ser 335. Taken together, our findings establish the molecular basis of phosphorylation-mediated regulation of ASBT which may include novel therapeutic targets for managing ASBT-linked disease.

2024-09-05 | PXD035472 | panorama

Quantifying forms and functions of intestinal bile acid pools in mice

Project description:Bile acids (BAs) are gastrointestinal metabolites that serve dual functions in lipid absorption and cell signaling. BAs circulate actively between the liver and distal small intestine (i.e., ileum), yet the dynamics through which complex BA pools are absorbed in the ileum and interact with intestinal cells in vivo remain ill-defined. Through multi-site sampling of nearly 100 BA species in individual wild type mice, as well as mice lacking the ileal BA transporter, Asbt/Slc10a2, we calculate the ileal BA pool in fasting C57BL/6J mice to be ~0.3 mmoles/g. Asbt-mediated transport accounts for ~80% of this pool and amplifies size, whereas passive absorption explains the remaining ~20%, and generates diversity. Accordingly, ileal BA pools in mice lacking Asbt are ~5-fold smaller than in wild type controls, enriched in secondary BA species normally found in the colon, and elicit unique transcriptional responses in cultured ileal explants. This work quantitatively defines ileal BA pools in mice and reveals how BA dysmetabolism can impinge directly on intestinal physiology.

2024-02-21 | GSE256113 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data