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Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist (ChIPseq, ATACseq)

ABSTRACT: Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist (ChIPseq, ATACseq)

PROVIDER: PRJNA546535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9203430.fastq.gz	Fastqsanger.gz
	SRR9203431.fastq.gz	Fastqsanger.gz
	SRR9203432.fastq.gz	Fastqsanger.gz
	SRR9203433.fastq.gz	Fastqsanger.gz
	SRR9203434_1.fastq.gz	Fastqsanger.gz

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Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist (ChIPseq, ATACseq)

Project description:Skeletal muscle possesses remarkable regenerative ability owing to the resident muscle stem cells (MuSCs). A prominent feature of quiescent MuSCs is a high content of heterochromatin. However, little is known about the mechanisms by which heterochromatin is maintained in MuSCs. We found that the mammalian Hairless (Hr) gene is expressed in quiescent MuSCs. Using a mouse model in which Hr can be specifically ablated in MuSCs, we demonstrate that Hr expression is critical for MuSC function and muscle regeneration. We show that Hr is a histone demethylase antagonist that preserves Histone 3 Lysine 9 trimethylation (H3K9me3) and maintains heterochromatin structure. Loss of Hr results in reduced H3K9me3 levels, reduced heterochromatin, increased susceptibility of MuSCs to genotoxic stress, and the accumulation of DNA damage. Our study not only elucidates the molecular mechanism by which Hr regulates histone demethylases, but also demonstrates the importance of heterochromatin maintenance in stem cell function.

2021-09-09 | GSE132220 | GEO

Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist

Project description:Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist

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Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist [RNA-seq]

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Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist [RNA-seq]

Project description:Hairless regulates heterochromatin maintenance and muscle stem cell function as a histone demethylase antagonist [RNA-seq]

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ATACSeq and genome-wide binding profile of EBF1 and Pax5 to unravel molecular networks driving leukemia in Ebf1+/-Pax5+/- (dHet) B-ALL mice.

Project description:ATACSeq, EBF1 ChIPSeq and Pax5 ChIPSeq of dHet B-ALL, dHet proB and wt proB cells.

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Expression changes in Hairless depleted Kc cells

Project description:Several co-repressors interact directly with the DNA-binding protein CSL [Su(H) in Drosophila] and are proposed to keep target genes silenced in the absence of Notch activity. To investigate co-repressor activity in the context of this well defined signalling pathway, we analysed the genome-wide binding profile of the best-characterized CSL co-repressor in Drosophila, Hairless, in Kc cells and in wing imaginal discs. The binding profile in wing discs of a second CSL interacting repressor, SMRTER, was also analysed. There was significant overlap between Hairless and Su(H), both in Kc cells and in wing discs, where they were predominantly found in chromatin with active enhancer marks. The Hairless complex was widely present at some Notch regulated enhancers in the wing disc,but no binding was detected at others, indicating that it is not essential for silencing per se. Analysis of target enhancers confirmed differential requirements for Hairless. SMRTER binding significantly overlapped with Hairless, rather than complementing it, and many enhancers were apparently co-bound by both factors. Our analysis indicates that the actions of Hairless and SMRTER gate the enhancers to Notch activity and to Ecdysone signalling respectively, to ensure that the appropriate levels and timing of target gene expression are achieved.

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Genomic profile of Hairless binding in Drosophila wing discs

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Genome-wide profile of Hairless occupancy in Kc cells

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Project description:ATACseq data for this study.

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Project description:AhR antagonist

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