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ABSTRACT: Transcriptome sequencing of Coccinella septempunctata adults

PROVIDER: PRJNA547368 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR9208150_1.fastq.gz	Fastqsanger.gz
	SRR9208150_2.fastq.gz	Fastqsanger.gz
	SRR9208141_1.fastq.gz	Fastqsanger.gz
	SRR9208141_2.fastq.gz	Fastqsanger.gz
	SRR9208151_1.fastq.gz	Fastqsanger.gz

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Project description:The most commonÂ ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing. The experimental insects consisted of three different states as follows: Non-diapause female insects were reared at 24Â±1Â°C, with a RH of 70Â±10% and a 16:8 h light: dark (L: D) photoperiod and collected after their first oviposition. Female adults in diapause were reared at 18Â±1Â°C at an RH of 70Â±10% and a 10:14-h (L:D) photoperiod. The experimental diapause insects were collected after 30 days. Diapause-terminated adults were transferred to another climatic cabinet with the 30-day diapause insects and reared under the same conditions as the non-diapause insects. After their first oviposition, the female insects were collected and stored at -80Â°C. Three biological replicates per treatment (non-diapause, diapause, diapause-terminated) were sequenced using Illumina HiSeq 2500.

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