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Transcriptome changes caused by Tudor-SN mutations in rice seed

ABSTRACT: Transcriptome changes caused by Tudor-SN mutations in rice seed

PROVIDER: PRJNA547574 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9211450_1.fastq.gz	Fastqsanger.gz
	SRR9211450_2.fastq.gz	Fastqsanger.gz
	SRR9211451_1.fastq.gz	Fastqsanger.gz
	SRR9211451_2.fastq.gz	Fastqsanger.gz
	SRR9211452_1.fastq.gz	Fastqsanger.gz

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Transcriptome changes caused by Tudor-SN mutations in rice seed

Project description:One OsTudor-SN mutant, EM1084, carrying E503K point mutation and one associated WT-OsTSN complemented transgenic (pHL8042/EM1084 ) were applied in this study. EM1084 was obtained by TILLING studies (http://www.shigen.nig.ac.jp/rice/oryzabase). The E503K mutation site in EM1084 lies within the SN3 domain, while the EM1084 complemented transgenic plant was complemented with the full-length WT OsTSN gene driven by native OsTSN promoter. This mutation results in affecting storage protein mRNA localization and, in turn, with a strong reduction in grain weight and storage protein accumulation. Transcriptome analysis on the dehulled 10-14 days old developing seeds from wild type, EM1084 and pHL8042/EM1084 indicates that partial loss of Tudor-SN function caused the differential expression of storage protein genes and relevant genes involved in several essential biological processes during rice development.

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Project description:Two RBP-P mutants, P1MH and P3MH, carrying G401S and A252T point mutations, respectively, were obtained by TILLING studies (http://www.shigen.nig.ac.jp/rice/oryzabase). The A252T mutation site in P3MH lies within the linker sequence between the two RRM domains while the G401S substitutions in P1MH is located in the glycine-rich C-terminal region. Those mutations results in varying degrees of reduced RNA binding and/or protein-protein interactive properties of RBP-P. Transcriptome analysis on the dehulled 10-14 days old developing seeds from wild type, P1MH and P3MH mutants indicates that partial loss of RBP-P function caused the differential expression of storage protein genes and relevant genes involved in several essential biological processes during rice development.

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