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Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq AtT-20 cells]

ABSTRACT: Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq AtT-20 cells]

PROVIDER: PRJNA547602 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9211860_1.fastq.gz	Fastqsanger.gz
	SRR9211860_2.fastq.gz	Fastqsanger.gz
	SRR9211861_1.fastq.gz	Fastqsanger.gz
	SRR9211861_2.fastq.gz	Fastqsanger.gz
	SRR9211852_1.fastq.gz	Fastqsanger.gz

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Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells

Project description:Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells

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Project description:Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [ChIP-Seq]

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Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq IL]

Project description:Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq IL]

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Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [ChIP-Seq]

Project description:Translation is a basic cellular process and its capacity is adapted to cell function. In particular, secretory cells achieve high protein synthesis levels without triggering the protein stress response. It is unknown how and when translation capacity is increased during differentiation. Here, we show that the transcription factor Creb3l2 is a scaling factor for translation capacity in pituitary secretory cells and that it directly binds ~75% of regulatory and effector genes for translation. In parallel with this cell-autonomous mechanism, implementation of the physiological UPR pathway prevents triggering the protein stress response. The pituitary differentiation factor Tpit activates Creb3l2 expression, the Creb3l2-dependent regulatory network as well as the physiological UPR pathway. Thus, Creb3l2 implements high basal translation levels through direct targeting of translation effector genes acting downstream of signaling pathways that otherwise regulate protein synthesis. Expression of Creb3l2 may be a useful means to enhance production of therapeutic proteins.

2019-07-29 | GSE132320 | GEO

Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq IL]

2019-07-29 | GSE132322 | GEO

Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [RNA-Seq AtT-20 cells]

2019-07-29 | GSE132321 | GEO

Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-07-29 | GSE132324 | GEO

Signaling from the secretory granule to the nucleus: Uhmk1 and PAM

Project description:Neurons and endocrine cells package peptides in secretory granules (large dense core vesicles; LDCVs) for storage and stimulated release. Studies of PAM, an essential secretory granule membrane enzyme, revealed a pathway that can relay information from secretory granules to the nucleus, resulting in alterations in gene expression. The cytosolic domain of PAM, a type 1 membrane enzyme essential for the production of amidated peptides, is basally phosphorylated by Uhmk1 and other Ser/Thr kinases. Proopiomelanocortin processing in AtT-20 corticotrope tumor cells was increased when Uhmk1 expression was reduced. Uhmk1 was concentrated in the nucleus, but cycled rapidly between nucleus and cytosol. Endoproteolytic cleavage of PAM releases a soluble cytosolic domain (CD) fragment that localizes to the nucleus. Localization of PAM-CD to the nucleus was decreased when PAM-CD with phosphomimetic mutations was examined and when active Uhmk1 was simultaneously over-expressed. Membrane-tethering Uhmk1 did not eliminate its ability to exclude PAM-CD from the nucleus, suggesting that cytosolic Uhmk1 could cause this response. Microarray analysis demonstrated the ability of PAM to increase expression of a small subset of genes, including aquaporin 1 (Aqp1) in AtT-20 cells. Aqp1 mRNA levels were higher in wildtype mice than in mice heterozygous for PAM, indicating that a similar relationship occurs in vivo. Expression of PAM-CD also increased Aqp1 levels while expression of Uhmk1 diminished Aqp1 expression. The outlines of a pathway that ties secretory granule metabolism to the transcriptome are thus apparent. Three separate clones of AtT-20 mouse corticotrope tumor cells stably transfected with doxycycline-inducible rat PAM-1 (clones 14, 6_1, 6_2) were treated with cotrol or doxycycline medium for 48 hours. Total mRNA was isolated and hybridized to an Illumina MouseWG-6 v 2.0 whole genome BeadChip.

2010-07-29 | E-GEOD-22509 | biostudies-arrayexpress

Signaling from the secretory granule to the nucleus: Uhmk1 and PAM

2010-06-23 | GSE22509 | GEO

The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity

Project description:Abstract: Secretion occurs in all cells, with relatively low levels in most cells and extremely high levels in specialized secretory cells, such as those of the pancreas, salivary and mammary glands. Here, we report that the CrebA/Creb3-like family of bZip transcription factors functions to upregulate expression of both the general protein machinery required in all cells for secretion and of cell-type specific secreted proteins. Drosophila CrebA directly binds the enhancers of secretory pathway genes and is both necessary and sufficient to activate expression of every secretory pathway component gene examined thus far. Microarray profiling reveals that CrebA also upregulates expression of genes encoding cell type specific secreted components. Finally, we find that the human CrebA orthologues, Creb3L1 and Creb3L2, have the ability to upregulate the secretory pathway in non-secretory cell types. This SuperSeries is composed of the following subset Series: GSE23334: Active Creb3L1 can upregulate secretory pathway genes in HeLa cells GSE23346: CrebA is a major and direct regulator of secretory pathway gene expression Refer to individual Series

2010-10-18 | E-GEOD-23349 | biostudies-arrayexpress

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