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MiRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME)

ABSTRACT: miRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME)

PROVIDER: PRJNA547723 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9214370.fastq.gz	Fastqsanger.gz
	SRR9214380.fastq.gz	Fastqsanger.gz
	SRR9214390.fastq.gz	Fastqsanger.gz
	SRR9214400.fastq.gz	Fastqsanger.gz
	SRR9214371.fastq.gz	Fastqsanger.gz

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miRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME)

Project description:We report the miRNA profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LPS/pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling either 72 h (RAW264.7) or 144 h (TIME). Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of either LPS (1 µg/mL; from E. coli serotype 0111:B4) for cell line RAW264.7 or the cytokines IL-1β, TNF-α, and IFN-γ each in a concentration of 5 ng/ml for cell line TIME.

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