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Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

ABSTRACT: Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

PROVIDER: PRJNA548077 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9257300_1.fastq.gz	Fastqsanger.gz
	SRR9257300_2.fastq.gz	Fastqsanger.gz
	SRR9257301_1.fastq.gz	Fastqsanger.gz
	SRR9257301_2.fastq.gz	Fastqsanger.gz
	SRR9257302_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

Project description:Analysis of the transcriptome of THP-1 cells upon Huh7 cell-derived ectosomes incubation. Monocytic THP-1 cells were incubated with or without ectosomes (10 mg/ml) derived from scramble sequence transfection (Scramble Ecto) or PKM2 knocking down (PKM2 KD Ecto) Huh7 cells for 24 h. Results provide a novel insight into monocyte differentiation.

2022-06-08 | GSE132435 | GEO

Transcriptome profiling of L02 cells upon Huh7-cell-derived large extracellular vesicles incubation

Project description:Analysis of the transcriptome of L02 cells upon Huh7-cell-derived large extracellular vesicles (lEVs) incubation. L02 cells were incubated with or without lEVs (10 μg/ml) derived from control or ACSL4 knocking down (ACSL4 KD) Huh7 cells for 3 days, and the gene expression profiling analysis were then performed. The result provides a new perspective on the crosstalk between Huh7 cells and L02 cells via lEV-mediated ACSL4 transmission.

2024-08-20 | GSE260856 | GEO

Transcriptome profiling of L02 cells upon Huh7-cell-derived large extracellular vesicles incubation

Project description:Transcriptome profiling of L02 cells upon Huh7-cell-derived large extracellular vesicles incubation

| PRJNA1083799 | ENA

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

Project description:Purpose: The goals of this study are to compare the gene expression profile of Huh7 cells treated with different ectosomes or not and to evaluate the function of ectosomal Hexokianse 1 (HK1) derived from HSCs. Methods: Firstly, we treated different LX-2 HSCs with TGF-β (2 ng/mL) for 36 hours to activate HSCs, then ectosomes from LX-2 or HK1-KD LX-2 culture medium were isolated respectively by differential centrifugation processes. Secendly, Huh7 cells were preincubated with different ectosomes or not for 12 hours and then fresh medium for another 12 hours. Lastly, different groups of Huh7 cells were prepared for RNA-seq. Results: By Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes, a total of 124 pathways were found to be enriched in the ectosome-incubated Huh7 cells compared with the control Huh7 cells, while a total of 36 pathways were enriched in the ectosome-incubated Huh7 cells compared with the HK1-KD ectosome-incubated Huh7 cells.Gene set enrichment analysis (GSEA) also revealed enrichment of the N-glycosylation pathway in the ectosome-incubated Huh7 cells compared with the control Huh7 cells. Conversely, this pathway was negatively regulated in Huh7 cells incubated with HK1-KD HSC-derived ectosomes Conclusion: the transferal of HSC-derived ectosomal HK1 into HCC cells regulates the N-glycosylation level of Huh7.

2024-04-28 | GSE173632 | GEO

Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:ATACseq analysis of chromatin accessibility in Huh7 cells upon YFV capsid expression

2020-11-15 | GSE152623 | GEO

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

Project description:RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

| PRJNA726435 | ENA

Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

Project description:Chromatin accessibility analysis of Huh7 cells upon YFV capsid expression

| PRJNA639846 | ENA

Expression data from CF proband and parent PBMCs; expression data from THP-1 monocytes/macrophages incubated with CF proband and parent plasma

Project description:We used microarrays to look at differential gene expression in PBMC samples from proband, proband's parents, and healthy controls We used microarrays to look at the differential gene expression in THP-1 monocyte/macrophages after incubation with plasma from CF probands, proband's parents, healthy controls

2021-12-24 | GSE192523 | GEO

RNAseq analysis of Huh7 cells upon stable-knockdown of L1 elements

Project description:RNAseq analysis of Huh7 cells upon stable-knockdown of L1 elements

| PRJNA522737 | ENA

Proteins secreted by M1 and M2 macrophages derived from human buffy coat or THP-1 cells

Project description:Proteins secreted into the conditioned medium by human monocytic cell line, THP-1, or human buffy coat-derived monocytes polarised to either an M1 (incubation for 48 h with 100 ng/mL LPS and 20 ng/mL IFN-gamma) or M2 (incubation for 48 h with 20 ng/mL IL-4) phenotype. The conditioned medium was enriched for anionic molecules (including proteoglycans) by anion exchange chromatography prior to analysis.

2021-01-21 | MSV000086746 | MassIVE

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