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Genome-wide maps of chromatin accessibility before and after NR2F2 knock down using ATAC-seq.

ABSTRACT: Genome-wide maps of chromatin accessibility before and after NR2F2 knock down using ATAC-seq.

PROVIDER: PRJNA548081 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9257303_1.fastq.gz	Fastqsanger.gz
	SRR9257303_2.fastq.gz	Fastqsanger.gz
	SRR9257304_1.fastq.gz	Fastqsanger.gz
	SRR9257304_2.fastq.gz	Fastqsanger.gz

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Genome-wide maps of chromatin accessibility before and after NR2F2 knock down using ATAC-seq.

Project description:FOXA1 and GATA3 can remodel chromatin accessibility, we further explored what effect of NR2F2 would have on chromatin properties. ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) is widely used to map chromatin accessibility genome-wide. Thus, We perform ATAC-seq without oestrogen stimulation before and after NR2F2 depletion.Covalent modifications are a main chromatin property.To test whether NR2F2 favoured histone modification deposition on chromatin, we profiled ChIP-Seq of H3K4me1, H3K4me3, and H3K27ac following NR2F2 depletion in oestrogen-starved MCF-7 cells to gain comprehensive histone medication landscape.

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