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Genome-wide maps of HDA6pro::HDA6:GFP in 14 days old arabidopsis

ABSTRACT: Genome-wide maps of HDA6pro::HDA6:GFP in 14 days old arabidopsis

PROVIDER: PRJNA548365 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9274082.fastq.gz	Fastqsanger.gz
	SRR9274083.fastq.gz	Fastqsanger.gz
	SRR9274084.fastq.gz	Fastqsanger.gz

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Genome-wide maps of HDA6pro::HDA6:GFP in 14 days old arabidopsis

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of HDA6 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide HDA6-binding maps of 14 days old arabidopsis. To reveal bound genes by HDA6, chimeric protein HDA6-GFP was expressed under HDA6 promoter in hda6 (HDA6pro:HDA6:GFP/ hda6). ChIP was performed using anti-GFP antibody (ab290; ABCAM), and ChIP DNA were analyzed by Illumina HiSeq 2500.

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Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3K4 demethylase LDL1 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide LDL1-binding maps of 14 days old arabidopsis. To reveal bound genes by LDL1, chimeric protein LDL1-GFP was expressed under LDL1 promoter in ldl1 (LDL1pro:LDL1:GFP/ ldl1). ChIP was performed using anti-GFP antibody (ab290; ABCAM), and ChIP DNA were analyzed by Illumina HiSeq 2500.

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