Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters

ABSTRACT: RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters

PROVIDER: PRJNA548379 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters

Project description:In mammalian cells, widespread acceleration of cytoplasmic mRNA degradation is linked to impaired RNA polymerase II (Pol II) transcription. This mRNA decay-induced transcriptional repression occurs during infection with gammaherpesviruses including Kaposi’s sarcoma associated herpesvirus (KSHV) and murine gammaherpesvirus 68 (MHV68), which encode an mRNA endonuclease that initiates widespread RNA decay. Here, we show that MHV68-induced mRNA decay leads to a genome-wide reduction of Pol II occupancy at mammalian promoters. Viral genes, despite the fact that they require Pol II for transcription, escape this transcriptional repression. Protection is not governed by viral promoter sequences; instead, location on the viral genome is both necessary and sufficient to escape the transcriptional repression effects of mRNA decay. We hypothesize that the ability to escape from transcriptional repression is linked to the localization of viral DNA in replication compartments, providing a means for these viruses to counteract decay-induced viral transcript loss.

2019-12-10 | PXD015786 | Pride

RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters.

Project description:In mammalian cells, widespread acceleration of cytoplasmic mRNA degradation is linked to impaired RNA polymerase II (Pol II) transcription. This mRNA decay-induced transcriptional repression occurs during infection with gammaherpesviruses including Kaposi's sarcoma-associated herpesvirus (KSHV) and murine gammaherpesvirus 68 (MHV68), which encode an mRNA endonuclease that initiates widespread RNA decay. Here, we show that MHV68-induced mRNA decay leads to a genome-wide reduction of Pol II occupancy at mammalian promoters. This reduced Pol II occupancy is accompanied by down-regulation of multiple Pol II subunits and TFIIB in the nucleus of infected cells, as revealed by mass spectrometry-based global measurements of protein abundance. Viral genes, despite the fact that they require Pol II for transcription, escape transcriptional repression. Protection is not governed by viral promoter sequences; instead, location on the viral genome is both necessary and sufficient to escape the transcriptional repression effects of mRNA decay. We propose a model in which the ability to escape from transcriptional repression is linked to the localization of viral DNA within replication compartments, providing a means for these viruses to counteract decay-induced transcript loss.

| S-EPMC7032723 | biostudies-literature

RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters

Project description:We perform RNA Polymerase II ChIp sequencing on MHV68 infected MC57G B6 mouse fibroblasts alongside a MHV68 mutant (R443I) that is deficient for RNA decay. We profile the occupancy of Pol II on host and viral genes at 24h post infection.We find that host genes have less Pol II occpancy genome wide, while viral genes remain robustly transcribed irrespective of RNA decay.

2019-06-12 | GSE132574 | GEO

Human gammaherpesvirus 8

Project description:Human gammaherpesvirus 8 Epigenomics

| PRJNA338021 | ENA

Genome-wide transcript structure resolution in murine gammaherpesvirus 68: Illumina RNA-Seq

Project description:Strand-specific Illumina RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68.

2019-06-25 | GSE117645 | GEO

Genome-wide transcript structure resolution in murine gammaherpesvirus 68: 3T12 BGI RNA-Seq

Project description:Strand-specific RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68.

2019-06-25 | GSE131198 | GEO

Comparative Analysis of Gammaherpesvirus Circular RNA

Project description:Comparative Analysis of Gammaherpesvirus Circular RNA

| PRJNA513195 | ENA

Genome-wide transcript structure resolution in murine gammaherpesvirus 68: HE 2.1 BGI RNA-Seq

Project description:Strand-specific RNA-Seq was used in conjunction with Pacific Biosciences Iso-Seq and deepCAGE to globally resolve transcript structures in lytic murine gammaherpesvirus 68.

2019-06-25 | GSE131199 | GEO

Human gammaherpesvirus 4

Project description:Human gammaherpesvirus 4 from Argentina

| PRJNA679281 | ENA

Human gammaherpesvirus 4

Project description:Human gammaherpesvirus 4 Genome sequencing

| PRJNA253520 | ENA