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Genome-wide maps of H3Ac in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

ABSTRACT: Genome-wide maps of H3Ac in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

PROVIDER: PRJNA548566 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9287832.fastq.gz	Fastqsanger.gz
	SRR9287833.fastq.gz	Fastqsanger.gz
	SRR9287834.fastq.gz	Fastqsanger.gz
	SRR9287835.fastq.gz	Fastqsanger.gz
	SRR9287836.fastq.gz	Fastqsanger.gz

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Genome-wide maps of H3Ac in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3Ac in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we mapped genome-wide H3AC levels of 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis. ChIP was performed using anti-H3K9K14Ac antibody (Millipore; 06-599), and ChIP DNA were analyzed by Illumina HiSeq 2500.

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Genome-wide maps of H3K4me2 in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3K4me2 in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we mapped genome-wide H3K4me2 levels of 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis. ChIP was performed using anti-H3K4me2 antibody (diagenode; C15410035), and ChIP DNA were analyzed by Illumina HiSeq 2500.

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RNA-seq of 14 days old arabidopsis in Col hda6 ldl1/2 and hda6/ldl1/2

Project description:We report the application ofr high-throughput profiling of total transcript in WT, hda6, ldl1/2 and hda6/ldl1/2. The total RNA were extracted and analysed by NGS to identify the differential expressed genes between WT with hda6, ldl1/2 and hda6/ldl1/2.

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Genome-wide maps of H3K4me2 in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

Project description:Genome-wide maps of H3K4me2 in 14 days old WT, hda6, ldl1/2 and hda6/ldl1/2 arabidopsis

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Genome-wide maps of LDL1pro::LDL1:GFP in 14 days old arabidopsis

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3K4 demethylase LDL1 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide LDL1-binding maps of 14 days old arabidopsis. To reveal bound genes by LDL1, chimeric protein LDL1-GFP was expressed under LDL1 promoter in ldl1 (LDL1pro:LDL1:GFP/ ldl1). ChIP was performed using anti-GFP antibody (ab290; ABCAM), and ChIP DNA were analyzed by Illumina HiSeq 2500.

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Project description:RNA-seq of 14 days old arabidopsis in Col hda6 ldl1/2 and hda6/ldl1/2

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