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Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

ABSTRACT: Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

PROVIDER: PRJNA548808 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9300303_1.fastq.gz	Fastqsanger.gz
	SRR9300303_2.fastq.gz	Fastqsanger.gz
	SRR9300304_1.fastq.gz	Fastqsanger.gz
	SRR9300304_2.fastq.gz	Fastqsanger.gz
	SRR9300305_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

Project description:A549 cells were co-transfected with CRISPR/Cas9 (containing sgRNA and GFP) and HDR donor plasmids (containing RFP and puromycin). The genetic editing cells were selected by puromycin. And then, we performed RNA sequencing for transcription profiling of A549 RB1 RB1 wild-type/knockout cell lines. The raw data were processed with Tophat and cuffdiff for gene expression analysis.

2020-08-25 | GSE132722 | GEO

Expression data of mRNA from wild-type A549 cells and microRNA-203 knockout A549 cells during H5N1 infections

Project description:MicroRNA-203 was up-regulated markedly upon H5N1 virus infection. To identify the potential target genes of miR-203, we constructed a miR-203 knockout A549 cell line. Then wild-type and miR-203 knockout A549 cells were mock-infected or infected with H5N1 virus for 48h. The Agilent Whole Human Genome Oligo Microarray was performed to analyze the mRNA expression profiling. Meanwhile, the online tool TargetScanHuman (http://www.targetscan.org/vert_71/) was used to predict biological targets of miR-203. We combined the predicted genes with the genes differentially expressed in wild-type and miR-203 knockout A549 cells, and preliminarily identified some candidate mRNAs. Then more experiments were performed to further verify these target genes, such as dual-luciferase reporter assay, quantitative real-time PCR or Western blot analysis.

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2024-12-18 | GSE216107 | GEO

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