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PRC1 catalytic activity is central to Polycomb system function. [ChIP]

ABSTRACT: PRC1 catalytic activity is central to Polycomb system function. [ChIP]

PROVIDER: PRJNA548922 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10327355_1.fastq.gz	Fastqsanger.gz
	SRR10327355_2.fastq.gz	Fastqsanger.gz
	SRR10327356_1.fastq.gz	Fastqsanger.gz
	SRR10327356_2.fastq.gz	Fastqsanger.gz
	SRR10327357_1.fastq.gz	Fastqsanger.gz

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PRC1 catalytic activity is central to Polycomb system function. [RNA]

Project description:The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how it selects its target genes is poorly understood and whether its histone modifying activities are required for transcriptional repression remains controversial. Here, we directly test the requirement for PRC1 catalytic activity in Polycomb system function. To achieve this, we develop a new inducible mutation system in embryonic stem cells that completely removes PRC1 catalytic activity. Using this system, we demonstrate that catalysis by PRC1 is important for Polycomb chromatin domain formation and long-range chromatin interactions. Furthermore, we show that variant PRC1 complexes with DNA-binding activities occupy target sites independently of Polycomb chromatin domain formation, providing a putative mechanism for Polycomb target site selection. Finally, we discover that Polycomb-mediated gene repression requires PRC1 catalytic activity. Together these discoveries provide compelling new evidence that PRC1 catalysis is central to Polycomb system function and gene regulation.

2020-01-08 | GSE132753 | GEO

PRC1 catalytic activity is central to Polycomb system function. [ChIP]

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PRC1 catalytic activity is central to Polycomb system function. [CaptureC ]

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PRC1 catalytic activity is central to Polycomb system function.

Project description:PRC1 catalytic activity is central to Polycomb system function.

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PRC1 catalytic activity is central to Polycomb system function. [CaptureC ]

Project description:PRC1 catalytic activity is central to Polycomb system function. [CaptureC ]

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PRC1 catalytic activity is central to Polycomb system function. [RNA]

Project description:PRC1 catalytic activity is central to Polycomb system function. [RNA]

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PRC1 Catalytic Activity Is Central to Polycomb System Function.

Project description:The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the Polycomb system selects its target genes is poorly understood, and whether its histone-modifying activities are required for transcriptional repression remains controversial. Here, we directly test the requirement for PRC1 catalytic activity in Polycomb system function. To achieve this, we develop a conditional mutation system in embryonic stem cells that completely removes PRC1 catalytic activity. Using this system, we demonstrate that catalysis by PRC1 drives Polycomb chromatin domain formation and long-range chromatin interactions. Furthermore, we show that variant PRC1 complexes with DNA-binding activities occupy target sites independently of PRC1 catalytic activity, providing a putative mechanism for Polycomb target site selection. Finally, we discover that Polycomb-mediated gene repression requires PRC1 catalytic activity. Together these discoveries provide compelling evidence that PRC1 catalysis is central to Polycomb system function and gene regulation.

| S-EPMC7033600 | biostudies-literature

PRC1 catalytic activity is central to Polycomb system function.

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-01-08 | GSE132754 | GEO

Polycomb repressive complex 1 (PRC1) role in skin epithelium development [HF]

Project description:Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification.

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