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Chromatin accessibility dynamics in a model of human forebrain development

ABSTRACT: Chromatin accessibility dynamics in a model of human forebrain development

PROVIDER: PRJNA549493 | ENA |

REPOSITORIES: ENA

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Chromatin accessibility dynamics in a model of human forebrain development

Project description:In this study, we used a forebrain 3D spheroid model to map chromatin accessibility and gene expression changes throughout a dense time course spanning 20 months of neuronal and glial maturation.

2020-01-23 | GSE132403 | GEO

Chromatin accessibility dynamics in a model of human forebrain development.

Project description:Forebrain development is characterized by highly synchronized cellular processes, which, if perturbed, can cause disease. To chart the regulatory activity underlying these events, we generated a map of accessible chromatin in human three-dimensional forebrain organoids. To capture corticogenesis, we sampled glial and neuronal lineages from dorsal or ventral forebrain organoids over 20 months in vitro. Active chromatin regions identified in human primary brain tissue were observed in organoids at different developmental stages. We used this resource to map genetic risk for disease and to explore evolutionary conservation. Moreover, we integrated chromatin accessibility with transcriptomics to identify putative enhancer-gene linkages and transcription factors that regulate human corticogenesis. Overall, this platform brings insights into gene-regulatory dynamics at previously inaccessible stages of human forebrain development, including signatures of neuropsychiatric disorders.

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Developmental dynamics of DNA accessibility during flower development [DNaseI-Seq]

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