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Knockdown of Znf148 by shRNA in MDA-MB-231 breast cancer cells.

ABSTRACT: Knockdown of Znf148 by shRNA in MDA-MB-231 breast cancer cells.

PROVIDER: PRJNA549521 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9323120.fastq.gz	Fastqsanger.gz
	SRR9323121.fastq.gz	Fastqsanger.gz
	SRR9323122.fastq.gz	Fastqsanger.gz
	SRR9323117.fastq.gz	Fastqsanger.gz
	SRR9323118.fastq.gz	Fastqsanger.gz

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Knockdown of Znf148 by shRNA in MDA-MB-231 breast cancer cells.

Project description:RNA-Sequencing of basal subtype triple negative breast cancer cells, MDA-MB-231. MDA-MB-231 cells were lentivirally transduced with pLKO short-hairpin (sh) luciferase control or shZnf148 in triplicates. Differentially expressed genes by the shRNA compared to the control were determined.

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Project description:We used MYOSIN10 shRNA to stably silence the expression of endogenous MYOSIN10 in Breast cancer cell MDA-MB-231. To investigate the inner change of cells with silenced MYOSIN10, we conducted a genome-wide screening for all potential genes affected by MYOSIN10 shRNA using Affymetrix Human Genome U133 plus 2.0 array. We showed genes affected by MYOSIN10 knockdown in breast cancer cell MDA-MB-231

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