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Oncogenic Regulatory Network Driven by IRF4 and NF-kappaB in Adult T-cell Leukemia/Lymphoma

ABSTRACT: Oncogenic Regulatory Network Driven by IRF4 and NF-kappaB in Adult T-cell Leukemia/Lymphoma

PROVIDER: PRJNA549785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7496555.fastq.gz	Fastqsanger.gz
	SRR7496556.fastq.gz	Fastqsanger.gz
	SRR9327790.fastq.gz	Fastqsanger.gz
	SRR9327791.fastq.gz	Fastqsanger.gz
	SRR9327792.fastq.gz	Fastqsanger.gz

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Oncogenic Regulatory Network Driven by IRF4 and NF-kappaB in Adult T-cell Leukemia/Lymphoma

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Project description:The NF-KappaB family of transcription factors plays a critical role in numerous cellular processes, particularly the immune response. Our understanding of how the different NF-kappaB subunits act coordinately to regulate gene expression is based on a limited set of genes. We used genome-scale location analysis to identify targets of all five NF-kappaB proteins before and after stimulation of monocytic cells with bacterial lipopolysaccharide (LPS). In unstimulated cells, p50 and p52 bound to a significant number of gene promoters. p50 occupied genes together with RNA polymerase II and defined a set of genes to which other NF-kappaB proteins bound after LPS induction. In stimulated cells, genes bound by multiple NF-kappaB subunits exhibited the greatest increases in RNA polymerase II occupancy and gene expression. This study identifies novel NF-kappaB target genes, reveals how the different NF-kappaB proteins coordinate their activity and maps transcriptional regulatory networks that underlie the host response to infection.

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