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Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA

ABSTRACT: Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA

PROVIDER: PRJNA550655 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9600570_1.fastq.gz	Fastqsanger.gz
	SRR9600570_2.fastq.gz	Fastqsanger.gz
	SRR9600580.fastq.gz	Fastqsanger.gz
	SRR9600561_1.fastq.gz	Fastqsanger.gz
	SRR9600561_2.fastq.gz	Fastqsanger.gz

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Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA [RNA-seq]

Project description:RNA-seq and RNAchIP-seq experiments were performed on MEF-derived cell lines knock-out for the expression of HP1g/CBX3 (R cell line) or recomplemented for the expression of HP1g (HPg cell line). Cells were tested under normal growth or conditions of stress (p samples) by treatment with the phorbol ester PMA. Each experiment was performed in biological triplicates.

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Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA [RNA-seq]

Project description:Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA [RNA-seq]

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Project description:Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA [RIP-seq]

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Genome-wide mapping of Cbx3/HP1g deposition in wild-type CD8+ effector T cells

Project description:We report the application of Illumina Hi-Seq sequencing technology for high-throughput profiling of Cbx3/HP1g deposition in mammalian CD8+ effector T cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, genome-wide chromatin-state maps of mouse wt CD8+ effector T cells were generated. We find that Cbx3/HP1g negatively regulates the germinal center and high-affinity antibody responses in a CD8+ T-cell-dependent manner. CD8-restricted deletion of Cbx3/HP1g confers hightened effector and persistence capacity on CD8+ effector T cells. As a result, Cbx3/HP1g-deficient CD8+ effector T cells can efficiently control solid tumor growth in vairious mouse models (melanoma, ovarian and neuroblstoma).

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Chromatin-associated RNAchIP and transcriptome analyses of the relationship between HP1g/CBX3 and RNA

Project description:This SuperSeries is composed of the SubSeries listed below.

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Genome-wide mapping of Cbx3/HP1g deposition in wild-type CD8+ effector T cells

Project description:Genome-wide mapping of Cbx3/HP1g deposition in wild-type CD8+ effector T cells

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Expression data from pre-iPSCs with a control, histone methyltransferase or Cbx3 (HP1g) knockdown

Project description:Transition from a partially reprogrammed pre-iPSC state to iPSC state can be achieved by modulating levels of histone modifying enzymes or proteins that can bind to histone modifications We used microarrays to determine the gene expression profile of pre-iPSCs depleted for either 3 histone methyltransferases together or the HP1gamma protein pre-iPSCs were subjected to simultaneous siRNA mediated knockdown of Ehmt1, Ehmt2 and Setdb1- which is the 3XHMT sample that mediate H3k9me2/me3 or Cbx3( HP1gamma) or control siRNA to luciferase

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2014-08-01 | E-GEOD-48897 | biostudies-arrayexpress

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