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Map of dimorphic switching-related signaling pathways in Sporothrix schenckii based on its transcriptome

ABSTRACT: Map of dimorphic switching-related signaling pathways in Sporothrix schenckii based on its transcriptome

PROVIDER: PRJNA551118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9602167_1.fastq.gz	Fastqsanger.gz
	SRR9602167_2.fastq.gz	Fastqsanger.gz
	SRR9602168_1.fastq.gz	Fastqsanger.gz
	SRR9602168_2.fastq.gz	Fastqsanger.gz

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Map of dimorphic switching-related signaling pathways in Sporothrix schenckii based on its transcriptome

Project description:Sporotrichosis, an implantation mycosis caused by the dimorphic fungus Sporothrix schenckii, gained attention over the two last decades due to its broad geographic range and prevalence in tropical and subtropical areas. The dimorphic switching from hyphal form to the yeast form is essential for the pathogenesis of S. schenckii. During the mycelium-to-yeast transition, there are many changes in asexual development, yeast-phase cell formation, cell wall integrity, and melanin synthesis. However, the mechanisms of the signaling pathways associated with these process remain unclear. In this study, transcriptome sequencing of both the yeast stage and mycelium of S. schenckii was carried out. The results showed that 12217 genes, including genes involved in signal transduction and chitin synthesis, were expressed differentially between the two stages. According to these results, a map of the signaling pathways, related to the dimorphic switch, was drawn. Overall, the transcriptome data and analysis presented here lay the foundation for further research into the molecular mechanisms controlling the dimorphic switch of S. schenckii and support the development of anti-S. schenckii strategies targeting genes related to signaling pathways.

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Map of dimorphic switching‑related signaling pathways in Sporothrix schenckii based on its transcriptome.

Project description:Sporothrix schenckii (S. schenckii) induces sporotrichosis, which has gained attention in recent years due to its worldwide prevalence. The dimorphic switching process is essential for the pathogenesis of S. schenckii. Previously, overexpression of several signal transduction genes, including SsDRK1 and SsSte20, was observed during the mycelium‑to‑yeast transition; these were necessary for asexual development, yeast‑phase cell formation, cell wall integrity and melanin synthesis. However, the mechanisms of the signaling pathways during dimorphic switching of S. schenckii remain unclear. In the present study, transcriptome sequencing of the 48‑h induced yeast forms and mycelium of S. schenckii was performed. In total, 24,904,510 high‑quality clean reads were obtained from mycelium samples and 22,814,406 from 48‑h induced yeast form samples. Following assembly, 31,779 unigene sequences were obtained with 52.98% GC content (The proportion of guanine G and cytosine C to all bases in nucleic acid). The results demonstrated that 12,217 genes, including genes involved in signal transduction and chitin synthesis, were expressed differentially between the two stages. According to these results, a map of the signaling pathways, including two‑component and heterotrimeric G‑protein signaling systems, Ras and MAPK cascades associated with the dimorphic switch, was drawn. Taken together, the transcriptome data and analysis performed in the present study lay the foundation for further research into the molecular mechanisms controlling the dimorphic switch of S. schenckii and support the development of anti‑S. schenckii strategies targeting genes associated with signaling pathways.

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Project description:We report the global transcriptomic differences occurring between mold and yeast phases of S. schenckii including changes in gene expression profiles associated with these distinct cellular phenotypes. Moreover, we also propose a new genome annotation which reveals a more complex transcriptional architecture than previously assumed.

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Ste20 is crucial for dimorphic switching of sporothrix schenckii and affects its global transcriptome.

Project description:Sporothrix schenckii induced sporotrichosis has gained importance in recent years because of its worldwide prevalence. The dimorphic switching process is required for the pathogenesis of S. schenckii. Previously, we found that STE20-like protein kinase (SsSte20) was overexpressed in the early yeast stage, but not in the mycelial stage of S. schenckii, which suggested its involvement in morphogenesis of this fungal pathogen. It remains unclear, however, whether SsSte20 is essential for dimorphic switching of S. schenckii and what are its related genes. In this study, the function of SsSte20 was investigated using double-stranded RNA interference (dsRNAi) mediated by Agrobacterium tumefaciens. We evaluated its effects on normal asexual development, yeast-phase cell formation, and cell wall composition and integrity. In addition, by transcriptome analysis of the SsSte20 knockdown (SsSte20-i) mutant and the standard S. schenckii strain, we further investigated the genes and pathways that were affected by SsSte20. Our results showed that inactivation of SsSte20 significantly affected the growth and internal components of S. schenckii conidia and impaired the dimorphic switching process. RNA transcriptome analysis of the standard S. schenckii strain and the SsSte20-i mutant revealed that SsSte20 inhibition affected the genes that were not only involved in the biological process, but also in the cellular component, and the molecular functions of S. schenckii. It mainly affected the expression of iron/ion-binding transporter genes, oxidation-reduction-related genes, 1, 3-beta-glucosidase, and methylsterol monooxygenase, which are highly associated with environmental information processing and the biosynthesis of cell wall components. Overall, our research supports the claim that SsSte20 plays an essential role in the dimorphism of S. schenckii and affects its global transcriptome.

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