Project description:Tri-methylation of histone H3 lysine 27 is catalyzed by Polycomb Repressive Complex 2 (PRC2). By genome-wide mapping of H3K27me3 sites in HEK293 cells, we are able to identify potential sites for PRC1-mediated repression and use these data to inform gene expression experiments in the presence and absence of pharmacological inhibition or KD/KO of various Polycomb subunits.
Project description:The Yin Yang 2 (YY2) gene encodes a zinc finger transcription factor that is not well characterized, yet. By using chromatin immunoprecipitations combined with whole-genome human promoter microarray (ChIP-chip) in HEK293 cells, we identified a multiplicity of YY2-bound annotated promoters as well as additional chromosomal regions. Interestingly, gene ontology analyses linked YY2 to fundamental biological pathways associated to cancer and developmental processes. Identification of YY2 target genes in HEK293 cells in vivo
Project description:This SuperSeries is composed of the following subset Series: GSE27967: ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [ChIP-seq] GSE27969: ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [mRNA profiling] Refer to individual Series
Project description:This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. It contains ChIP-chip data on Agilent 244K dual-color arrays for antibody: H3K27me3 at 12 different time-points of Drosophila development. Current Dataset: [GSM384769..GSM384777]: ChIP-chip of H3K27me3 in Drosophila embryos at 0-4 hours of development [GSM384778..GSM384786]: ChIP-chip of H3K27me3 in Drosophila embryos at 4-8 hours of development [GSM384787..GSM384795]: ChIP-chip of H3K27me3 in Drosophila embryos at 12-16 hours of development [GSM384797..GSM384805]: ChIP-chip of H3K27me3 in Drosophila embryos at 16-20 hours of development [GSM384807..GSM384815]: ChIP-chip of H3K27me3 in Drosophila embryos at 20-24 hours of development [GSM384817..GSM384825]: ChIP-chip of H3K27me3 in Drosophila L1 larvae [GSM384826..GSM384834]: ChIP-chip of H3K27me3 in Drosophila L2 larvae [GSM384839..GSM384847]: ChIP-chip of H3K27me3 in Drosophila pupae [GSM385341..GSM385349]: ChIP-chip of H3K27me3 in Drosophila L3 larvae [GSM418225..GSM418233]: ChIP-chip of H3K27me3 in Drosophila Adult male [GSM418239..GSM418247]: ChIP-chip of H3K27me3 in Drosophila embryos at 8-12 hours of development [GSM442006..GSM442014]: ChIP-chip of H3K27me3 in Drosophila Adult female For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf For each combination of time-point and antibody, triplicate ChIP experiments have been performed and hybridized on Agilent 244K arrays. 3 arrays per genome have been used so that each time-point is a set of 9 tiling arrays.