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Alterations in alternative RNA splicing in hepatocytes from high-fat diet fed C57BL/6 male mice

ABSTRACT: Alterations in alternative RNA splicing in hepatocytes from high-fat diet fed C57BL/6 male mice

PROVIDER: PRJNA551398 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9606504_1.fastq.gz	Fastqsanger.gz
	SRR9606504_2.fastq.gz	Fastqsanger.gz
	SRR9606505_1.fastq.gz	Fastqsanger.gz
	SRR9606505_2.fastq.gz	Fastqsanger.gz
	SRR9606506_1.fastq.gz	Fastqsanger.gz

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Alterations in alternative RNA splicing in hepatocytes from high-fat diet fed C57BL/6 male mice

Project description:C57BL/6J male mice were fed a 60% HFD for 10 weeks, then hepatocytes were isolated, RNA extracted and subjected to RNAseq

2020-04-13 | GSE133400 | GEO

Global hepatic transcript data from fed male BXD strains on chow or high fat diet

Project description:Gene expression levels assessed by microarray in liver samples collected from the BXD mouse genetic diversity model fed either a standard chow or a high fat diet at a fed state.

2022-09-27 | GSE188764 | GEO

Expression data from livers of C57BL/6J mice fed a high-fat diet and coffee

2013-12-10 | GSE53131 | GEO

Expression data from livers of C57BL/6J mice fed a high-fat diet and coffee

Project description:The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. The present study was conducted to evaluate the effects of the intake of three types of coffee (caffeinated, decaffeinated, and green unroasted coffee) on the livers of C57BL/6J mice fed a high-fat diet, and to extensively elucidate the physiological responses to coffee intake by analysing the findings obtained from a comprehensive transcriptomic analysis using DNA microarrays. Briefly, 7-week-old male C57BL/6J mice purchased from Charles River Laboratories Japan (Yokohama) were divided into the following five groups. The normal diet group (ND group) was fed D12450B (10 kcal% fat, Research Diets, New Brunswick, NJ, USA). The high-fat diet group (HF group) was fed D12492 (60 kcal% fat, Research Diets, New Brunswick, NJ, USA). The caffeinated coffee group (HFCC group) was fed a high-fat diet containing 2% caffeinated freeze-dried coffee. The decaffeinated coffee group (HFDC group) was fed a high-fat diet containing 2% decaffeinated freeze-dried coffee. The green unroasted coffee group (HFGC group) was fed a high-fat diet containing 2% unroasted caffeinated freeze-dried coffee. The mice had ad libitum access to their diets and drinking water. After 9 weeks, mice were sacrificed and the livers were subjected to the Affymrtix DNA microarray experiment.

2013-12-10 | E-GEOD-53131 | biostudies-arrayexpress

Transcriptomic analysis of primary hepatocytes and total livers from WT mice fed with CSAA or CDAA-HSHC diet during 2 weeks.

Project description:Mouse primary hepatocytes (PH) and total livers (TL) prepared from C57Bl6 male adult mice which were fed either a CDAA supplemented cholesterol with glucose and fructose or a CSAA diet during 2 weeks.

2024-04-01 | GSE212617 | GEO

Gene expression data of livers from C57BL/6 fed a normal diet or high-fat-diet

Project description:Obesity is tightly associated with an increased risk of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms of obesity-induced fatty liver remain largely unknown.In order to identify genes that are potentially involved in dysfunctional hepatic lipid homeostasis in obesity, we performed a clustering analysis of Affymetrix arrays,which revealed that a number of mRNAs were dys-regulated in the livers of mice fed a high-fat diet (HFD), compared with mice fed a normal chow diet (ND). To identify genes that are potentially involved in dysfunctional hepatic lipid homeostasis in obesity, male C57BL/6 mice aged 8 weeks were fed a normal diet (ND) or high-fat-diet (HFD) containing 60 Kcal% of fat for 12 weeks. Then mice were sacrificed and total RNAs were isoloated from hepatic tissues. Affymetrix array hybridisation and scanning were performed using Mouse Genome 430 2.0 chips.Total RNA samples obtained from six mice per group (ND and HFD) and pooled by each of the two were used for microarray analysis.

2014-05-09 | E-GEOD-57425 | biostudies-arrayexpress

Spleen proteomics data from high fat diet fed mice

Project description:The composition of the diet affects many processes in the body, including body weight and endocrine system. We have previously shown that dietary fat also affects the immune system. Mice fed high fat diet rich in polyunsaturated fatty acids survive S. aureus infection to a much greater extent than mice fed high fat diet rich in saturated fatty acids. Here we present data regarding the dietary effects on protein expression in spleen from mice fed three different diets, I) low fat/chow diet (LFD, n=4), II) high fat diet rich in saturated fatty acids (HFD-S, n=4) and III) high fat diet rich in polyunsaturated fatty acids (HFD-P, n=4). We performed mass spectrophotometry based quantitative proteomics analysis of isolated spleen by implementing the isobaric tags for relative and absolute quantification (iTRAQ) approach. Mass spectrometry data were analysed using Proteome Discoverer 2.4 software using the search engine mascot against Mus musculus in SwissProt. 924 proteins are identified in all sets (n=4) for different dietary effects taken for statistical analysis using Qlucore Omics Explorer software. Only 20 proteins were found to be differentially expressed with a cut-off value of false discovery rate < 0.1 (q-value) when comparing HFD-S and HFD-P but no differentially expressed proteins were found when LFD was compared with HFD-P or HFD-S. We identified a subset of proteins that showed an inverse expression pattern between two high fat diets. These differentially expressed proteins were further classified by gene ontology for their role in biological processes and molecular functions.

2020-08-06 | PXD020365 | Pride

Age- and BAM15-related remodeling of inguinal white adipose tissue in high fat diet-fed mice

Project description:Aged (~92 weeks of age) C57BL/6J mice and young mice at 16 weeks of age were fed 60% HFD for 4 weeks containing either vehicle or 0.1% w/w BAM15. iWAT was harvested and snap frozen in liquid nitrogen. Tissue was homogenized in trizol and RNA extracted via chloroform phase separation.

2023-11-25 | GSE162231 | GEO

Myocardial expression data from ketogenic diet-fed mice

Project description:Specific pathogen free wild-type C57Bl/6 male mice fed ketogenic diet (Bio-Serv AIN-76-A) for 4 weeks Keywords: RNA Expression Array

2010-06-07 | GSE21368 | GEO

Single cell RNA-seq of liver cells from B6J mice fed a chow or a high-fat-and-high-sucrose diet

Project description:Cannabinoid receptor-1 (CB-1) blockage in brain is responsible for weight loss, many of the metabolic benefits associated with CB-1 blockade have been attributed to inhibition of CB-1 signaling in the periphery. We produced mice that lacked CB-1 receptors in hepatocytes or stellate cells to determine if CB-1 signaling contributes to the development of NAFLD or liver fibrosis. Deletion of CB-1 receptors in hepatocytes did not alter the development of NAFLD in mice fed a high sucrose high fat diet (HSD) or high fat diet (HFD). Similarly, deletion of CB-1 deletion specifically in stellate cells also did not prevent the development of NAFLD in mice fed the HFD nor did it protect mice for carbon tetrachloride-induced fibrosis. To determine if a small population of cells in liver express CB-1 at high levels either before and/or after HSD feeding, here we performed single cell RNA-sequencing of livers from wild-type mice fed chow or a HSD for 17 weeks and analyzed CB-1 expression. Single-cell sequencing of hepatocytes and stellate cells reveals low Cnr1 expression in livers of mice fed a chow or HSD. Combined, these studies do not support a direct role for hepatocyte or stellate cell CB-1 signaling in the development of NAFLD or liver fibrosis.

2021-09-01 | GSE182365 | GEO

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