Project description:The objective of this study was to evaluate the effect of the oomycete Aphanomyces euteiches (strain ATCC201684) on the legume model Medicago truncatula (F83005-5 line) transcritpome.
Project description:Inoculation of Medicago truncatula roots with zoospores of the oomycetic pathogen Aphanomyces euteiches leads to rapid upregulation of a gene encoding a sesquiterpene synthase followed by release of sesquiterpenes that may have a defense function against A. euteiches.
Project description:This experiment captures the gene expression data from pea roots during interaction with two pathogenic oomycetes: Phytophthora pisi and Aphanomyces euteiches, at 6 hours and 20 hours after infection and the control samples at each time point. In this experiment medicago genome array is used.
Project description:affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula – Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed.