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Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (RNAseq data set)

ABSTRACT: Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (RNAseq data set)

PROVIDER: PRJNA552040 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9623120.fastq.gz	Fastqsanger.gz
	SRR9623121.fastq.gz	Fastqsanger.gz
	SRR9623122.fastq.gz	Fastqsanger.gz
	SRR9623123.fastq.gz	Fastqsanger.gz
	SRR9623124.fastq.gz	Fastqsanger.gz

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Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans

Project description:Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans

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Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (Microarray expression)

Project description:Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (Microarray expression)

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Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (Microarray expression)

Project description:Candida albicans is an opportunistic pathogen which is responsible for widespread nosocomial infections. It encompasses a fungus specific serine/threonine protein phosphatase gene CaPPZ1 that is involved in cation transport, cell wall integrity, oxidative stress response, morphological alterations, and virulence. We demonstrated that tert-butyl-hydoperoxide induced oxidative stress suppressed the growth rate and blocked the proliferation of the fungal cells without effecting their viability, both in the cappz1 KO mutant and in the genetically matching QMY23 control strains. To reveal the gene expression changes behind the above observation we carried out a global transcriptome analysis by DNA microarray hybridization as well as RNA sequencing, and confirmed our results by quantitative RT-PCR. The numbers of genes affected and the amplitudes of the transcript-level changes revealed that the deletion of CaPPZ1 enhances the effects of oxidative stress. Thus the roles of this fungus specific protein phosphatase in important physiological processes like membrane transport, cell surface interactions, oxidoreductase activity, cell signaling, translation, and RNA processing becomes more pronounced under oxidative conditions. We conclude that in the wild type C. albicans CaPPZ1 has a protective role against oxidative damage, and suggest that the specific inhibition of this phosphatase combined with mild oxidative treatment could be a feasible approach of topical antifungal therapy.

2019-11-04 | GSE133335 | GEO

Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans (RNAseq data set)

2019-11-04 | GSE133611 | GEO

Gene expression changes of tert-butyl alchol-induced in renal cortex of rats

Project description:This study is to develop a short term and highly accurate prediction method of renal carcinogenicity based on gene expression profile of rats administrated by carcinogens. We conducted 28 days-repeated dose experiments in male SD rats with tert-butyl alchol, and the gene expression profiles of renal cortex were analyzed using custom microarrays.

2016-09-27 | GSE87278 | GEO

The effect of tert-butyl hydroperoxide on hepatic multi-transcript patterns of the sentinel fish Lithognathus mormyrus

Project description:The study was aimed at examining the effect of the model organic oxygen reactive species (ROS) tert-Butyl Hydroperoxide (tBHP) on hepatic multi-gene expression patterns of the fish Lithognathus mormyrus. Fish were exposed to injected tBHP followed by extraction of RNA from their livers. The corresponding labeled cDNAs of treated versus control fish were applied onto a cDNA microarray of ~1500 unique sequences and differentially expressed genes were identified. The hepatic profile of the in individual fish versus a reference RNA was the basic biomarker parameter. M, the log2 ratio of gene expression (M) was calculated from the imaged hybridization results by the LIMMA software (R environment) used also to statistically determine the differentially expressed genes in the various treatments. EXPANDER4.1 software was used to hierarchically cluster M profiles of individual fish across genes. This clustering was used as a preliminary step aimed at distinguishing groups of similarly expressed genes across the treatments. Gene annotation was aimed at more educated interpretation of the results.

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Ribosomal/nucleolar stress induction regulates tert-butyl hydroperoxide (tBHP) mediated oxidative stress in Anopheles gambiae midguts

Project description:A general understanding of redox homeostasis in An. gambiae midgut epithelial cells under different oxidative conditions during P. falciparum ookinete invasion is missing. We used an ex vivo midgut culture model to understand AgTrx-1 protein expression pattern in An. gambiae midguts under oxidative stress of the ROS inducer, tert-butyl hydroperoxide (tBHP). We then quantified the midgut proteomic expression profile to understand organ-level regulation of the response to oxidative stress. An. gambiae midguts under low (50M) and high (200M) tBHP concentrations were enriched in ribosomal proteins (RPs), consistent with cells undergoing ribosomal/nucleolar stress. Nevertheless, these midguts were also enriched in peptidases, integral membrane proteins, and cytochrome P450s indicating cells that are undergoing protein processing and folding through post-translational modification (PTMs), and detoxification of toxic compounds through active efflux. This response to oxidative stress was strikingly dissimilar to that observed from previous studies with another dipteran, Drosophila following oxidative stress induction. Our data indicates that the response to tBHP induced oxidative stress is mild and suggests that the associated antioxidant response is similar to what is observed during midgut invasion of P. falciparum okinete. Furthermore, our data shows that ribosomal/nucleolar stress is crucial in the regulation of oxidative stress in An. gambiae midguts. Given this importance, its possible to develop mechanisms to perturb this ribosomal/nucleolar stress response in An. gambiae resulting into unmanageable levels of oxidative stress exposure to Plasmodium parasite in the midgut, yet still allowing the mosquito to survive the perturbation. Unmanageable levels of oxidative stress could result to harmful effect to the Plasmodium and eventually its death resulting into halting of its transmission.

2018-11-21 | PXD008251 | Pride

Gene expression changes of 4,4'-thiobis(6-tert-butyl-m-cresol)-induced in renal cortex of rats

Project description:This study is to develop a short term and highly accurate prediction method of renal carcinogenicity based on gene expression profile of rats administrated by carcinogens. We conducted 28 days-repeated dose experiments in male SD rats with 4,4'-thiobis(6-tert-butyl-m-cresol), and the gene expression profiles of renal cortex were analyzed using custom microarrays.

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Expression data from zebrafish (Danio rerio) embryos exposed to methyl tert-butyl ether

Project description:Methyl tert-butyl ether (MTBE) has been shown to target developing vasculature in piscine and mammalian model systems. In the zebrafish, MTBE induces vascular lesions throughout development. These lesions result from exposure to MTBE at an early stage in development (6-somites to Prim-5 stages). During this time period, transcript levels of vegfa, vegfc, and vegfr1 were significantly decreased in embryos exposed to 5 mM MTBE. We performed global gene analysis as an unbiased approach to discover possible modes of action of MTBE vascular toxicity.

2013-09-01 | GSE46371 | GEO

Deletion of the fungus specific protein phosphatase Z1 exaggerates the consequences of oxidative stress elicited by tert-butyl-hydroperoxide in Candida albicans

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-11-04 | GSE134060 | GEO