Project description:Vastus Lateralis muscle biopsies were obtained before and after the training protocol from 6 healthy elderly male people. We chose custom TaqMan low density array cards (Life Technologies) preloaded with 48 selected genes related to the following pathways: myogenesis, apoptosis, protein anabolism/catabolism and antioxidant enzymes to quantitate their gene expression in human skeletal muscle.
Project description:Needle biopsies were obtained from the vastus lateralis muscle of 6 healthy, sedentary, 67±2.5 year-old males before and after 3 months of training. Keywords: other
Project description:The molecular level the different muscular signature from Veteran soccer Players (VPG) vs age-matched active healthy subjects (control group, CG) was explored by using a proteomic approach. V. lateralis muscle biopsies were investigated in order to highlight the molecular mechanisms that characterize a successful aging through a lifelong football training
Project description:Changes in Gene exporession after 8 weeks of PrimaVie Shilajit Supplementation were measured in vastus lateralis GeneChip® Human Transcriptome Array 2.0 was used to study the gene expression in human vastus lateralis after supplementation of PrimaVie Shilajit and identified distinct classes of up-regulated genes during this process.
Project description:The effect of a long-term calorie restricted diet was evaluated in vastus lateralis muscle of rhesus monkeys For biopsy collection, the animals were given buprenorphine (0.01-0.03 mg/kg, IM) in the morning immediately preceding all biopsies and again that afternoon
Project description:To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition. Two-condition experiment, Vastus lateralis muscle biopsy post-expedition vs Vastus lateralis muscle biopsy pre-expedition. Five volunteers/climbers. Biological replicates: 1 pre-expedition sample, 1 post-expedition sample.
Project description:To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition.
Project description:Four healthy human volunteers underwent an acute bout of resistance exercise with the right leg at 2 pm. Biopsies were removed from the Vastus Lateralis muscle 6 h (8 pm) and 18 h (8 am) after exericise
Project description:This series includes the global gene expression profile of the vastus lateralis muscle for 10 young (19-25 years old) and 12 older (70-80 years old) male subjects.