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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

ABSTRACT: An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

PROVIDER: PRJNA552350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9636680.fastq.gz	Fastqsanger.gz
	SRR9636681.fastq.gz	Fastqsanger.gz
	SRR9636682.fastq.gz	Fastqsanger.gz
	SRR9636683.fastq.gz	Fastqsanger.gz
	SRR9636684.fastq.gz	Fastqsanger.gz

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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

Project description:The male terminalia of Drosophila melanogaster has undergone rapid morphological change between it and its closely related species, making it a useful model for studying evolution and development. In order to identify the network components that pattern the male terminalia, we isolated and sequenced whole RNA from two timepoints during pupal development, and next performed in situ hybridization for 100 highly expressed transcription factors during pupal development.

2019-12-10 | GSE133732 | GEO

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster.

Project description:During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have identified network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster male terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the male terminalia are highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (https://flyterminalia.pitt.edu/) as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.

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Terminalia arjuna

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Comparative transcriptome analyses of the Drosophila pupal eye

Project description:Investigating Drosophila melanogaster genome-wide gene expression differences in developing pupal eyes comparing 21 hours After puparium formation (APF) to 40 hours APF.

2020-10-30 | GSE160441 | GEO

Terminalia arjuna

Project description:Terminalia arjuna Genome sequencing

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Expression data from D. melanogaster pupal wings

2013-02-01 | GSE43673 | GEO

Expression data from D. melanogaster pupal wings

Project description:The goal of this gene expression profiling experiment was to identify the entire set of transcription factors expressed during late pupal wing development (~80h APF) when pigmentation genes are expressed We used Affymetrix microarrays to profile the expression of the transcription factors in late pupal wings of Oregon R flies. The staging of the flies was made by eye, based on the extent of wing pigmentation. RNA was extracted from batches of 40 flies and three biological replicates were analyzed.

2013-02-01 | E-GEOD-43673 | biostudies-arrayexpress

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