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Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

ABSTRACT: Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

PROVIDER: PRJNA552547 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9641320.fastq.gz	Fastqsanger.gz
	SRR9641321.fastq.gz	Fastqsanger.gz
	SRR9641322.fastq.gz	Fastqsanger.gz
	SRR9641323.fastq.gz	Fastqsanger.gz
	SRR9641324.fastq.gz	Fastqsanger.gz

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Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

Project description:Despite the well-reported association between H3K4me3 and transcription, ablation of H3K4me3 by deletion of SPP1 has little effect on nascent transcription.

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Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

Project description:Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

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N-terminal acetylation of the Set1-COMPASS fine-tunes H3K4 methylation patterns (ChIPseq for shg1 and spp1 deletion strains)

Project description:H3K4 methylation is a conserved histone modification crucial for gene regulation, yet the post-translational modifications of the Set1-COMPASS complex remain largely unexplored. This study elucidates the significance of N-terminal acetylation in modulating H3K4 methylation patterns. Firstly, loss of NatA complex resulted in a significant decrease in H3K4me3 levels and a shift of H3K4me2 from 5' transcribed regions to promoters. Importantly, NatA physically interacted with the Set1-COMPASS complex and facilitated N-terminal acetylation of the Shg1 subunit. Surprisingly, deletion of SHG1 or mutation of the acetylation site (A2P) in Shg1 led to reduced H3K4 methylation in cells lacking Spp1. Additionally, NatB complex also contributed to H3K4 methylation regulation, potentially through N-terminal acetylation of Swd1. These findings highlight the novel role of N-terminal acetylation in fine-tuning the function of the Set1-COMPASS complex and shaping H3K4 methylation patterns. This study sheds light on the intricate regulation of H3K4 methylation and emphasizes the significance of N-terminal acetylation as a regulatory mechanism in this process.

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Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

Project description:We report a drastic genome-wide reduction of H3K4me3 levels following deletion of SPP1.

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Context Dependency of Set1/COMPASS Mediated H3 Lysine 4 Trimethylation

Project description:The stimulation of trimethylation of histone H3 lysine 4 (H3K4) by H2B monoubiquitination (H2Bub) has been widely studied with multiple mechanisms proposed for this form of histone crosstalk. Cps35/Swd2 within COMPASS is considered to bridge these processes. However, a truncated form of Set1 (762-Set1) is reported to function in H3K4 trimethylation without interacting with Cps35/Swd2, and such crosstalk is attributed to the n-SET domain of Set1 and its interaction with the Cps40/Spp1 subunit of COMPASS. Here, we use biochemical, structural, in vivo, and ChIP-seq approaches to demonstrate that Cps40/Spp1 and the n-SET domain of Set1 are required for the stability of Set1 and not the crosstalk. Furthermore, the apparent wild-type levels of H3K4 trimethylation (H3K4me3) in the 762-Set1 strain is due to rogue methylase activity of this mutant resulting in the mislocalization of H3K4me3 from the promoter-proximal regions to gene bodies and intergenic regions. We have also performed detailed screens and identified yeast strains lacking H2Bub, but containing intact H2Bub enzymes, that have normal levels of H3K4me3, suggesting that ubiquitination may not directly stimulate COMPASS, but rather works in a context of the PAF and Rad6/Bre1 complexes. Our study demonstrates that the ubiquitination machinery and Cps35/Swd2 function to focus COMPASS’ H3K4me3 activity at promoter-proximal regions in a context dependent manner. ChIP-Seq for H3K4ME3 in S. cerevisie wild-type strains and strains expressing a truncated form of Set1: aa762-1080 Set1. H3K4ME3 ChIP-Seq was also compared for wild-type, leo1 knockout, and chd1 knockout strains

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Project description:N-terminal acetylation of the Set1-COMPASS fine-tunes H3K4 methylation patterns (ChIPseq for shg1 and spp1 deletion strains)

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Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation

Project description:Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation

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