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Analysis of genes regulated by miRNAs upon Toll pathway activation in shmCherry and shAgo1 flies

ABSTRACT: Analysis of genes regulated by miRNAs upon Toll pathway activation in shmCherry and shAgo1 flies

PROVIDER: PRJNA552858 | ENA |

REPOSITORIES: ENA

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Analysis of genes regulated by miRNAs upon Toll pathway activation in shmCherry and shAgo1 flies

Project description:This micro-array helps establish Toll activated-genes dampened by miRNAs in Drosophila melanogaster.

2024-06-05 | GSE133877 | GEO

Bulk RNAseq of Anopheles gambiae hemocytes upon Toll pathway overactivation

Project description:Bulk RNAseq of Anopheles gambiae hemocytes upon Toll pathway overactivation

| PRJEB49311 | ENA

Bulk RNAseq of Anopheles gambiae hemocytes upon Toll pathway overactivation

Project description:Toll pathway is a key mediator of antiplasmodial immunity and its mechanism of action is dependent on hemocytes (mosquito white blood cells). Anopheles gambiae were injected with dsRNA for Cactus, an inhibitor of the Toll pathway. Cactus silencing over activates the Toll pathway. The goal of this experiment is to evaluate hemocyte transcriptional changes between control mosquitoes injected with a non-related dsRNA (dsLacZ and dsCactus injected mosquitoes. We also divide hemocytes from control and experimental conditions into 2 groups: bound (hemocytes that attach to glass - granulocyte populations) and unbound (hemocytes that remain in suspension - prohemocytes and oenocytoids). Files from Unbound fractions are labelled: UB and from bound fractions are labelled B. Some of the samples were run twice and have 2 gz files.

2021-12-16 | E-MTAB-11252 | biostudies-arrayexpress

Overexpression of IBIN (CG44404) using C564-GAL4 driver enhances a group of Toll pathway target genes and shows effects on metabolism

Project description:We performed whole transcriptome profiling of adult flies overexpressing IBIN (Induced By INfection, CG44404) construct with the C564-GAL4 driver and control flies (IBIN;w1118) with and without infection with eiher E. cloacae (6h) or M. luteus (24h). Expression of a group of Toll pathway target genes upon M. luteus infection is slightly elevated when IBIN is overexpressed compared to controls. Importantly, cluster analysis of up- and downregulated genes in C564>IBIN flies without infection revealed two major gene clusters, both of which are involved in metabolism. 8/45 upregulated genes upon IBIN overexpression belong to a carbohydrate metabolism/glycoside hydrolase gene cluster, and 6/21 downregulated genes belong to a proteolysis / peptidase S1 gene cluster. Genes that had a normalized read number >10 in the treatment of interest and an expression fold change >2 were included in the cluster analysis performed with DAVID Bioinformatics resources 6.8 (https://david.ncifcrf.gov) online tool.

2019-01-01 | GSE120437 | GEO

Bombardier mediates delivery of short-form Bomanins in the Drosophila Toll response

Project description:Toll mediates a robust and effective innate immune response across vertebrates and invertebrates. In Drosophila melanogaster, activation of Toll by systemic infection drives the accumulation of a rich repertoire of immune effectors in hemolymph, including the recently characterized Bomanins as well as the classical antimicrobial peptides (AMPs). Here we report the functional characterization of a Toll-induced hemolymph protein encoded by the bombardier (CG18067) gene. Using the CRISPR-Cas9 system to generate a precise deletion, we found that Bombardier is required for Toll-mediated defense against fungi and Gram-positive bacteria. Assaying cell-free hemolymph from these flies, we found that the Bomanin-dependent candidacidal activity observed in hemolymph is also dependent on Bombardier, but not on the antifungal AMPs Drosomycin and Metchnikowin. Using mass spectrometry, we demonstrated that deletion of Bombardier results in the specific absence of short-form Bomanins from hemolymph. Flies lacking Bombardier also exhibited a defect in pathogen tolerance that we trace to an autoimmune disorder triggered by Toll activation. These results lead us to a model in which the presence of Bombardier in wild-type flies enables the proper folding, secretion, or intermolecular associations of short-form Bomanins, and the absence of Bombardier disrupts one or more of these steps, resulting in defects in both immune resistance and tolerance.

2019-10-25 | MSV000084509 | MassIVE

MYCN regulated miRNAs

Project description:MYCN regulated miRNAs

| PRJEB6787 | ENA

MYCN regulated miRNAs

Project description:miRNAs which are differentially expressed upon MYCN overexpression (1h, 4h and 24h after induction) in a neuroblastoma line were detected using deep sequencing.

2015-07-24 | E-MTAB-2787 | biostudies-arrayexpress

Drosophila lncRNA-NANPI enhances Toll signaling pathway by interacting with Dif/Dorsal to facilitate transcription of antibacterial peptides

Project description:LncRNAs have important regulatory functions but their roles in Drosophila innate immunity are poorly understood. The Drosophila Toll signaling pathway responds to Gram-positive bacterial infection and is highly conserved with mammalian TLR signaling. Recent studies focused mainly on Toll signaling pathway regulation by protein-coding genes. In the present study, we found that lncRNA-CR33942 was upregulated after Micrococcus luteus infection. Gain-of-function and loss-of-function assays disclosed that lncRNA-CR33942 regulates the Toll signaling pathway and affects Drosophila survival. RNA-seq of infected CR33942-overexpressing flies was performed to explore the CR33942 mechanism. About 70% of all upregulated core enrichment genes in the Toll signaling pathway were antibacterial peptides and PGRPs transcribed by Dif/Dorsal. We also demonstrated CR33942 interactions with Dif/Dorsal and revealed that they induce antibacterial peptides. Hence, we renamed CR33942 as lncRNA-NANPI (NF-κB-associated non-coding RNA promotional immunology). The present study identified the novel Toll signaling pathway regulator NANPI, elucidated its mode of action, clarified the function of lncRNA, and expanded our understanding of the Toll signaling pathway.

2021-05-07 | GSE174007 | GEO

Comparative microarray analysis of psh and Dif mutant flies treated with Metarhizium anisopliae ARSEF 549

Project description:The Toll pathway is the chief antifungal pathway in Drosophila. Two components of this pathway have disparate effects on Drosophila infected with Metarhizium ansiopliae ARSEF 549 (Ma549). Infected Drosophila Dif mutants show no change in susceptibility while infected psh mutants rapidly succumb to infection. We evaluated the impact of Ma549 on gene regulation in flies deficient in these Toll pathway components to identify the cause for these susceptibility differences. Four fly lines were used: psh, BDSC6326 (psh control), Dif, and cn bw (Dif control). RNA was collected 46 hrs post infection for 10 infected or uninfected male flies per replicate.

2022-08-31 | E-MTAB-9527 | biostudies-arrayexpress

Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals

Project description:Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals

| PRJNA159933 | ENA