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EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE EMBRYO

ABSTRACT: EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE EMBRYO

PROVIDER: PRJNA553226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9655240_1.fastq.gz	Fastqsanger.gz
	SRR9655240_2.fastq.gz	Fastqsanger.gz
	SRR9655250_1.fastq.gz	Fastqsanger.gz
	SRR9655250_2.fastq.gz	Fastqsanger.gz
	SRR9655241_1.fastq.gz	Fastqsanger.gz

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EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE EMBRYO

Project description:The effects of a high BUN on blastocoele fluid urea concentrations and analyzed the transcriptome of day-14 equine embryos based upon RNA sequencing.

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EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE ENDOMETRIUM AND EMBRYO

Project description:EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE ENDOMETRIUM AND EMBRYO

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EFFECT OF ORAL UREA ADMINISTRATION ON THE TRANSCRIPTOME OF THE EQUINE ENDOMETRIUM AND EMBRYO

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EFFECT OF ORAL UREA SUPPLEMENTATION ON THE ENDOMETRIAL TRANSCRIPTOME OF MARES

Project description:At ovulation detection (D0), oral treatment with urea was initiated and continued until D7. Mares received a treatment or control diet (n= 11 mares/group) in a crossover design. The treated group received urea (0.4 g/kg body weight) mixed with sweet feed and molasses, the control group received sweet feed and molasses alone. Blood samples were collected daily, one hour after feeding, for BUN determination. Uterine and vaginal pH were evaluated with an epoxy pH probe. Endometrial biopsies were taken transcervically one hour after the last feeding on D7. RNA sequencing of the endometrium of a subset of mares (n=6/group) was conducted.

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The equine embryo influences immune related gene expression in the oviduct

Project description:While the equine oviduct clearly affects early embryo development and while the selective transport of equine embryos through the oviduct indicates a reciprocal interaction, the influence of the embryo on gene expression in the oviduct remains to be determined in the horse. The aim of this study was to examine this by means of RNA sequencing. Four days after ovulation, the oviduct epithelial cells ipsilateral and contralateral to the ovulation side from five cyclic and five pregnant mares were collected. mRNA was extracted and samples were sequenced using the Illumina Hiseq-2000 sequencer. Data analysis was performed with the CLC Genomics software and differentially expressed genes (DEGs) were determined (p-value ≤ 0.05 and absolute fold change ≥ 2). ClueGO was used for functional interpretation. A total of 26,991 genes was identified and 253 genes were found to be upregulated and 108 to be downregulated in the pregnant ipsilateral oviduct, when compared to the cyclic ipsilateral oviduct. Comparison of the ipsilateral and the contralateral oviduct indicated 164 DEGs in pregnant mares and 77 DEGs in cyclic mares. Functional enrichment analysis only detected differences in the comparison of pregnant and cyclic ipsilateral oviducts and showed that the equine embryo affects the expression of immune response related genes in the oviduct, with marked upregulation of interferon associated genes. This research represents the foundation for further assessment of the role of specific genes in the early embryo-maternal dialogue of the horse.

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EFFECT OF ORAL UREA SUPPLEMENTATION ON THE ENDOMETRIAL TRANSCRIPTOME OF MARES

Project description:EFFECT OF ORAL UREA SUPPLEMENTATION ON THE ENDOMETRIAL TRANSCRIPTOME OF MARES

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urea-Urea impact on the nitrogen metabolism of plant and on the nitrogen transport systems

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