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ABSTRACT: Whole genome sequencing of three bromate reducing bacteria strains isolated from activated sludge

PROVIDER: PRJNA553302 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR10808204_1.fastq.gz	Fastqsanger.gz
	SRR10808204_2.fastq.gz	Fastqsanger.gz
	SRR10808205_1.fastq.gz	Fastqsanger.gz
	SRR10808205_2.fastq.gz	Fastqsanger.gz

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Project description:Bromate reducing microorganisms

| PRJNA649669 | ENA

Effects of TiO2 and Ag nanoparticles on polyhydroxybutyrate biosynthesis by activated sludge bacteria

Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.

2014-12-03 | GSE63781 | GEO

Effects of TiO2 and Ag nanoparticles on polyhydroxybutyrate biosynthesis by activated sludge bacteria

2014-12-03 | E-GEOD-63781 | biostudies-arrayexpress

Functional gene expressions of continuously cultured Nitrosomonas europae after ZnO nanoparticles exposure and recovery incubation

2016-06-24 | GSE83683 | GEO

Transcriptional expressions of Nitrosomonas europae after CeO2 nanoparticle exposure and 12-h recovery

Project description:To further explore the biotoxicity mechanisms of CeO2 nanoparticles (NPs) and the recovery strategies of the according impaired Nitrosomonas europaea (N. europaea, ATCC 19718) cells, a genome-sequenced model ammonia oxidizing bacterium (AOB) commonly detected in the activated sludge of biological wastewater treatment plants, the whole-genome microarray analysis was applied to retrieve the induced transcriptional responses, after their physiological and metabolic activities were evealed.

2018-11-28 | GSE122972 | GEO

activated sludge metagenome

Project description:Activated sludge bacteria Genome sequencing

| PRJNA295647 | ENA

Functional gene expressions of continuously cultured Nitrosomonas europae after ZnO nanoparticles exposure and recovery incubation

Project description:To further explore the biotoxicity mechanisms of zinc oxide nanoparticles (ZnO NPs) and the recovery strategies of the accordingly impaired Nitrosomonas europaea (N. europaea, ATCC 19718) cells, a genome-sequenced model ammonia-oxidizing bacterium (AOB) commonly detected in the activated sludge of biological wastewater treatment plants, whole-genome microarray analysis was applied to retrieve the induced transcriptional responses, after their physiological and metabolic activities were revealed. The whole-genome expressions were measured after exposure to 50 ppm ZnO NPs and 12-hrs recovery incubation when the ammonia removal rate (ARA) declined by 10% in the chemostat bioreactor. Three independent experiments were performed for each experiment.

2016-06-24 | E-GEOD-83683 | biostudies-arrayexpress

Transcriptional responses of continuously cultured Nitrosomonas europae to chonic TiO2 nanoparticle stress

Project description:To further explore the biotoxicity mechanisms of TiO2 nanoparticles (NPs) and the recovery potentials of the impaired Nitrosomonas europaea (N. europaea, ATCC 19718) cells, a genome-sequenced model ammonia oxidizing bacterium (AOB) commonly detected in the activated sludge of biological wastewater treatment plants, the whole-genome microarray analysis was applied to retrieve the induced transcriptional responses during the long-term exposure, after the toxicity effects and the recovery potentials were assessed at both physiological and metabolic levels.

2018-02-27 | GSE111100 | GEO

Combined effects of ZnO and TiO2 nanoparticles on functional gene expression of Nitrosomonas europaea

Project description:To further explore and differentiate the biotoxicity mechanisms of individual nanoparticles (NPs) and NP mixture on Nitrosomonas europaea (N. europaea, ATCC 19718) at genetic level, a genome-sequenced model ammonia oxidizing bacterium (AOB) commonly detected in the activated sludge of biological wastewater treatment plants, the induced whole-genome expressions were analyzed with the high throughput Microarray technique, after the dose-dependent changes of N. europaea’s physiological, metabolic and AMO enzyme activities in single and dual component NP systems was evaluated.

2015-06-23 | GSE70121 | GEO

Acinetobacter oleivorans DR1 : Wild-type cell harboring plasmid (pAST2) in nutrient media

Project description:Transcriptional profiling of A. oleivorans DR1 cells harboring pAST2. Plasmid pAST2 is a tetracycline-resistance plasmid which was isolated from activated sludge (Hong et al., 2014). The complete plasmid sequence was deposited in the National Center for Biotechnology Information (NCBI) GenBank under accession number KC734561 [PMID: 24337108].

2014-02-22 | GSE55239 | GEO

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