Project description:We performed a single-cell transcriptome analysis of 3 biological independent splenic samples from pooled mice sorted for LCMV-specific memory CD4 T cells.
Project description:To understand tissue resident features of memory CD4+ and CD8+ T lymphocytes of the bone marrow and/or spleen according to expressing or not the tissue retention marker CD69, we performed whole transcriptome profiling of ex vivo antigen-specific CD69+ and CD69- memory CD4+ T cells isolated from bone marrow and spleen, and ex vivo CD69+ and CD69- memory CD8+ T cells isolated from bone marrow.
Project description:We performed a ATAC seq analysis of 2 biological independent splenic samples from pooled mice sorted for lcmv-specific memory CD4 T cells and 2 technical replicates from one mouse sorted for naive CD4 T cells .
Project description:Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and colon
Project description:Single cell transcriptomic analysis (InDrops) of mouse FoxP3- CD4+ Tconv cells and FoxP3+ regulatory CD4+ T cells isolated from spleen and visceral adipose tissue
Project description:We report the differential mRNA expression of memory-like CD4+ T cells stimulated with iL-7, IL-1β, and IL-23. We found memory-like CD4+ T cells stimulated with IL-1β, or with IL-1β and IL-23, in the presence of IL-7, displayed a unique gene expression signature.