Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Brettanomyces (Dekkera)

ABSTRACT: Brettanomyces (Dekkera) species WGS Sequencing and assembly

PROVIDER: PRJNA554210 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	VKGP01.dat.gz	Other
	VKGP01.fasta.gz	Fasta.gz
	VKGP01.master.dat	Other
	SRR9679954_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Brettanomyces (Dekkera) species Sequencing and assembly

Project description:Genome comparison of the five strains of Brettanomyces (Dekkera) NCBI BioProject: PRJNA554210

| PRJEB33581 | ENA

Dekkera bruxellensis

Project description:Dekkera bruxellensis and Brettanomyces custersianus Raw sequence reads

| PRJNA335438 | ENA

Brettanomyces

Project description:Gene expression profiles in the two Brettanomyces/Dekkera yeasts

| PRJNA335567 | ENA

Molecular detection and identification of Brettanomyces/Dekkera bruxellensis and Brettanomyces/Dekkera anomalus in spoiled wines.

Project description:In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the DNA from these two species were used. No amplification product was obtained when DNA from other Brettanomyces spp. or wine yeasts were used as the templates. The 305-bp product was subjected to restriction enzyme analysis with DdeI to differentiate between B. bruxellensis and B. anomalus, and each species could be identified on the basis of the different restriction profiles. After optimization of the method by using strains from international collections, wine isolates were tested with the method proposed. Total agreement between traditional identification and molecular identification was observed. The protocol developed was also used for direct detection of B. bruxellensis and B. anomalus in wines suspected to be spoiled by Brettanomyces spp. Application of culture-based and molecular methods led us to the conclusion that 8 of 12 samples were spoiled by B. bruxellensis. Results based on the application of molecular methods suggested that two of the eight positive samples had been infected more recently, since specific signals were obtained at both the DNA and RNA levels.

| S-EPMC368351 | biostudies-literature

Brettanomyces bruxellensis LAMAP2480

Project description:Brettanomyces bruxellensis LAMAP2480 Genome sequencing and assembly

| PRJNA231184 | ENA

Brettanomyces anomalus strain:YV396

Project description:Brettanomyces anomalus strain:YV396 Genome sequencing and assembly

| PRJNA281311 | ENA

Brettanomyces bruxellensis CBS 2499

Project description:Brettanomyces bruxellensis CBS 2499 Genome sequencing and assembly

| PRJNA76499 | ENA

Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis.

Project description:The hemiascomycete yeast Dekkera bruxellensis, also known as Brettanomyces bruxellensis, is a major cause of wine spoilage worldwide. Wines infected with D. bruxellensis develop distinctive, unpleasant aromas due to volatile phenols produced by this species, which is highly ethanol tolerant and facultatively anaerobic. Despite its importance, however, D. bruxellensis has been poorly genetically characterized until now. We performed genome survey sequencing of a wine strain of D. bruxellensis to obtain 0.4x coverage of the genome. We identified approximately 3,000 genes, whose products averaged 49% amino acid identity to their Saccharomyces cerevisiae orthologs, with similar intron contents. Maximum likelihood phylogenetic analyses suggest that the relationship between D. bruxellensis, S. cerevisiae, and Candida albicans is close to a trichotomy. The estimated rate of chromosomal rearrangement in D. bruxellensis is slower than that calculated for C. albicans, while its rate of amino acid evolution is somewhat higher. The proteome of D. bruxellensis is enriched for transporters and genes involved in nitrogen and lipid metabolism, among other functions, which may reflect adaptations to its low-nutrient, high-ethanol niche. We also identified an adenyl deaminase gene that has high similarity to a gene in bacteria of the Burkholderia cepacia species complex and appears to be the result of horizontal gene transfer. These data provide a resource for further analyses of the population genetics and evolution of D. bruxellensis and of the genetic bases of its physiological capabilities.

| S-EPMC1865652 | biostudies-literature

Brettanomyces bruxellensis

Project description:Brettanomyces bruxellensis Raw sequence reads

| PRJNA281577 | ENA

Brettanomyces bruxellensis

Project description:Brettanomyces bruxellensis Raw sequence reads

| PRJNA318157 | ENA