Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomics of AML12 transfected with PAV-TBG-P2A-GFP-Chrna4 over-expression plasmid

ABSTRACT: Transcriptomics of AML12 transfected with PAV-TBG-P2A-GFP-Chrna4 over-expression plasmid

PROVIDER: PRJNA554633 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9684000.fastq.gz	Fastqsanger.gz
	SRR9684001.fastq.gz	Fastqsanger.gz
	SRR9684002.fastq.gz	Fastqsanger.gz
	SRR9684003.fastq.gz	Fastqsanger.gz
	SRR9684004.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Transcriptomics of AML12 transfected with PAV-TBG-P2A-GFP-Chrna4 over-expression plasmid

Project description:We reported the transcriptomics responses from RNA-seq of AML12 transfected with Chrna4 over-expression plasmid. We used cultured AML12 from 3 to 5 generations and transfected it with pCMV6-AC-GFP or PAV-TBG-P2A-GFP-Chrna4 plasmid. After 24 h, total RNA of cultured AML12 was extracted with RNAiso Plus (TaKaRa). Then we delegated it to Novogene company for RNAseq aiming to identify key factors promoting this transition.

2022-07-15 | GSE134274 | GEO

Effect of DUSP6 overexpression or knockdown on gene expression in AML12 cells

Project description:DUSP6 plays important roles in MAPK signaling pathway, but whether and how it is involved in liver funciton remains to be explored. Here, we performed RNA-seq analyses in AML12 cells where DUSP6 is disrupted. We overexpressed GFP-DUSP6 or GFP in AML12 cells, and tested the effects of DUSP6 increase on gene expression in AML12 cells. Meanwhile, we knocked down DUSP6 in AML12 cells and tested the effects of DUSP6 decrease on gene expression in AML12 cells. Taken together, we analyzed the changes of gene expression mediated by DUSP6, which provides new insights for the function of DUSP6 in liver

2023-12-31 | GSE249146 | GEO

Transcription profiling of mouse AML12 hepatocytes transfected with v-erbA

Project description:The v-erbA oncogene belongs to a superfamily of transcription factors called nuclear receptors, which includes the retinoic acid receptors (RARs) responsible for mediating the effects of retinoic acid (RA). Nuclear receptors bind to specific DNA sequences in the promoter region of target genes and v-erbA is known to exert a dominant negative effect on the activity of the RARs. The repressor activity of v-erbA has been linked to the development of hepatocellular carcinoma (HCC) in a mouse model. We have used microarray analysis to identify genes differentially expressed in hepatocytes in culture (AML12 cells) stably transfected with v-erbA and exposed to RA. We have found that v-erbA can affect expression of RA-responsive genes. We have also identified a number of v-erbA-responsive genes that are known to be involved in carcinogenesis and which may play a role in the development of HCC. Experiment Overall Design: AML12 control cells and v-erbA-transfected AML12 cells were exposed to 1 ÂµM RA for 3h or 24h. Using microarray analysis, we compared gene expression in the presence and absence of v-erbA and identified RA-regulated genes differentially expressed in the presence of v-erbA.

2008-10-20 | E-GEOD-11703 | biostudies-arrayexpress

DNA methylation data from AML12 cells during EMT

Project description:We investigated genome-wide DNA methylation during EMT in AML12 cells using comprehensive high throughput arrays for relative methylation (CHARM). We used custom Nimblegen microarrays. We isolated genomic DNA from cells at different timepoints post-TGF-β stimulation and hybridized to custom-designed Nimblegen microarrays (CHARM arrays). AML12 cells from 4 representative experiments were collected and used for the genome-wide DNA methylation analysis.

2011-07-03 | E-GEOD-27968 | biostudies-arrayexpress

Gene expression in the liver of mice infected with Fst288-AAV-TBG

Project description:We compared RNA expression profiles of wild type of mice maintained on high fat diet or Irs1/2:foxo1-LTKO mice infected with Fst288 AAV-TBG virus Fst288-AAV-TBG infection modestly up- or down-regulated the expression of many genes, including several related to hepatic glucose metabolism (Pck1, G6pc and Gck) (Fig. S8a); qPCR confirmed that Fst288AAV•TBG affected the expression of these and other genes during both fasting (Pck1, G6pc and Gck) and feeding (Foxo1, Igfbp1, Pck1, and Ppargc1a)

2018-03-14 | GSE111745 | GEO

Genome-wide maps of chromatin state in AML12 cells [ChIP-Seq]

Project description:We performed chromatin Immunoprecipitation (ChIP) assay followed by next-generation sequencingwith H3K9me3 and H3K27ac antibodies in WT AML12 cells to investigate the epigenetic landscape of AML12. And we performed ChIP-seq using H3K27me3 antibody in WT and MATR3-depleted AML12 cells.

2023-06-28 | GSE200241 | GEO

Gene expression analysis of wildtype AML12 versus VWA8-null AML12 cells

Project description:To obtain a more complete picture of gene expression changes induced by deletion of VWA8 null cells, Affymetrix microarray analysis of gene expression was carried out using RNA isolated from wildtype and VWA8-null AML12 cells.

2020-08-03 | GSE109610 | GEO

Gene expression analysis of wildtype AML12 versus VWA8-null AML12 cells

Project description:Gene expression analysis of wildtype AML12 versus VWA8-null AML12 cells

| PRJNA431472 | ENA

Genome-wide maps of chromatin state in AML12 cells [SAMMY-Seq]

Project description:We performed SAMMY (Sequential Analysis of Macro-Molecules accessibilitY) chromatin fraction assay followed by next-generation sequencing in WT and MATR3-depleted AML12 cells to investigate the epigenetic landscape of AML12.

2023-06-28 | GSE228141 | GEO

HEK cells expressing miRFP680-P2A-GFP, emiRFP670-P2A-GFP, emiRFP703-P2A-GFP, miRFP713-P2A-GFP and miRFP720-P2A-GFP

Project description:HEK cells transiently transfected with pAAV-CAG-NIR-FPs-P2A-GFP plasmids were processed using Mini MS Sample Prep kit according to the manufacture protocol. Extracted peptides were analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) in data-independent acquisition (DIA) mode. We reported the protein identity and relative concentration of NIR FPs and GFPs for each analyzed sample as well as post-translational modifications if any are identified.

2023-06-07 | PXD042781 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data