Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human oral metagenome

ABSTRACT: In vitro oral biofilms exposed to chlorhexidine

PROVIDER: PRJNA554992 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9697100_1.fastq.gz	Fastqsanger.gz
	SRR9697100_2.fastq.gz	Fastqsanger.gz
	SRR9697110_1.fastq.gz	Fastqsanger.gz
	SRR9697110_2.fastq.gz	Fastqsanger.gz
	SRR9697120_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

human oral metagenome

Project description:Chlorhexidine-free mouthwash inhibits pathobionts while preserving the oral microbiome

| PRJNA858625 | ENA

human oral metagenome

Project description:Alcohol and tobacco consumption affects bacterial richness in oral cavity biofilms

| PRJNA255332 | ENA

Real-time quantitative PCR analysis of human gingiva epithelial cells exposed to microbial biofilms

Project description:An organotypic gingiva model RHG was constructed by immortalized human gingiva cell lines (keratinocytes and fibroblasts) in vitro. The RHG model was exposed to either control medium or one of the three multi-species oral biofilms (commensal, gingivitis and cariogenic) for 24 hours. We used Qiagen RT² Profiler PCR array to analyse the expression of genes which are related to Toll-like receptor signaling pathway from the unexposed (control) and differently exposed (test) keratinocyte layer of the RHG model. Reference for RHG model and oral biofilms culture: J. K. Buskermolen et al., J Dent Res 97 (2018): 201-208. doi: 10.1177/0022034517729998.

2019-07-23 | GSE133422 | GEO

human oral metagenome

Project description:Development and pyrosequencing analysis of an in-vitro oral biofilm model

| PRJNA271580 | ENA

Evidence for proline utilisation by oral bacterial biofilms grown in saliva

Project description:Within the mouth bacteria are starved of saccharides as their main nutrient source between meals and it is unclear what drives their metabolism. Previously oral in vitro biofilms grown in saliva have shown proteolytic degradation of salivary proteins and increased extracellular proline. Although arginine and glucose have been shown before to have an effect on oral biofilm growth and activity, there is limited evidence for proline. Nuclear magnetic resonance (NMR) spectroscopy was used to identify extracellular metabolites produced by bacteria in oral biofilms grown on hydroxyapatite discs. Biofilms were inoculated with whole mouth saliva and then grown for 7 days using sterilised whole mouth saliva supplemented with proline, arginine and glucose as a growth-medium. Overall proline had a beneficial effect on biofilm growth – with significantly fewer dead bacteria present by biomass and surface area of the biofilms (p <0.05). Where arginine and glucose significantly increased and decreased pH, respectively, the pH of proline supplemented biofilms remained neutral at pH 7.3-7.5. SDS-polyacrylamide gel electrophoresis of the spent saliva from proline and arginine supplemented biofilms showed inhibition of salivary protein degradation of immature biofilms. NMR analysis of the spent saliva revealed that proline supplemented biofilms were metabolically similar to unsupplemented biofilms, but these biofilms actively metabolised proline to 5-aminopentanoate, butyrate and propionate, and actively utilised glycine. This study shows that in a nutrient limited environment, proline has a beneficial effect on in vitro oral biofilms grown from a saliva inoculum.

2020-07-23 | ST001433 | MetabolomicsWorkbench

human oral metagenome

Project description:Co-culture of oral bacteria using a novel in-vitro biofilm method

| PRJNA295539 | ENA

human oral metagenome

Project description:Gene expression anlayses of saliva-derived in vitro biofilms during carbohydrate fermentation and pH stress

| PRJNA264728 | ENA

human oral metagenome

Project description:Substrate-formed in situ biofilms mimic supragingivally tooth-formed biofilms

| PRJNA431137 | ENA

human oral metagenome

Project description:Oral mycobiome across stages of oral carcinogenesis